Effective Cell Separation Utilizing Poly(N-Isopropylacrylamide)-Grafted Polypropylene Membrane Containing Adsorbed Antibody(MEDICAL BIOTECHNOLOGY)
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概要
- 論文の詳細を見る
We previously developed a cell separation method using a poly(N-isopropylacrylamide)-grafted polypropylene(PNIPAAm-g-PP) membrane containing an adsorbed monoclonal antibody(mAb). The purpose of this study is to elucidate the cell separation mechanism in detail and to design an optimal method. As the grafting yield of PNIPAAm increased, the level of the adsorption of IgG_1 and cell adhesion to the membrane decreased. After BSA was adsorbed to a PNIPAAm-g-PP membrane at 6℃, where PNIPAAm was hydrophilic, a small amount of IgGt was adsorbed to the membrane at 37℃, where PNIPAAm was hydrophobic. The desorption of the adsorbed IgGj was not enhanced even though temperature was reduced to 10℃, where PNIPAAm was hydrophilic. These results indicate that the antibody adsorbed to the intact PP surface of the membrane predominantly contributes to the capture of target cells through the antigen-antibody reaction and that a thermoresponsive transition of PNIPAAm contributes to the detachment of the captured cells. The total number of cells recovered from a PNIPAAm-g-PP membrane containing the adsorbed mAb decreased as the grafting yield increased. A PNIPAAm-g-PP membrane with a 1.7% grafting yield containing adsorbed anti-human CD34 mAb enriched CD34-positive KG-1a cells to 85% from a 1:1 cell suspension of KG-1a cells and CD34-negative Jurkat cells.
- 2008-03-25
著者
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Okamura Aiko
Research Center of Advanced Bionics, National Institute of Advanced Industrial Science and Technolog
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Hagiwara Taeko
Research Center of Advanced Bionics, National Institute of Advanced Industrial Science and Technolog
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Yamagami Souko
Research Center of Advanced Bionics, National Institute of Advanced Industrial Science and Technolog
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Yamaguchi Manae
Research Center of Advanced Bionics, National Institute of Advanced Industrial Science and Technolog
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Shinbo Toshio
Research Center of Advanced Bionics, National Institute of Advanced Industrial Science and Technolog
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Kanamori Toshiyuki
Research Center of Advanced Bionics, National Institute of Advanced Industrial Science and Technolog
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Kondo Satoshi
New Frontiers Research Laboratories, Toray Industries, Inc.
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Miwa Keishi
New Frontiers Research Laboratories, Toray Industries, Inc.
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Itagaki Ichiro
New Frontiers Research Laboratories, Toray Industries, Inc.
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Miwa Keishi
New Frontiers Research Laboratories Toray Industries Inc.
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Okamura Aiko
Research Center Of Advanced Bionics National Institute Of Advanced Industrial Science And Technology
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Shinbo Toshio
Research Center Of Advanced Bionics National Institute Of Advanced Industrial Science And Technology
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Hagiwara Taeko
Research Center Of Advanced Bionics National Institute Of Advanced Industrial Science And Technology
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Itagaki Ichiro
New Frontiers Research Laboratories Toray Industries Inc.
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Yamagami Souko
Research Center Of Advanced Bionics National Institute Of Advanced Industrial Science And Technology
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Yamaguchi Manae
Research Center Of Advanced Bionics National Institute Of Advanced Industrial Science And Technology
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Kanamori Toshiyuki
Research Center Of Advanced Bionics National Institute Of Advanced Industrial Science And Technology
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Kondo Satoshi
New Frontiers Research Laboratories Toray Industries Inc.
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KANAMORI Toshiyuki
Research Center for Stem Cell Engineering (SCRC), National Institute of Advanced Industrial Science and Technology (AIST)
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