Enhanced Accumulation of Anthocyanin in Cultured Strawberry Cells by Repetitive Feeding of L-Phenylalanine into the Medium(Plant Biotechnology)
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概要
- 論文の詳細を見る
L-Phenylalanine (Phe), biosynthetic precursor of anthocyanin, was repetitively added into a suspension culture of strawberry cells, constantly producing anthocyanins. In the repetitive feeding culture, the maximum anthocyanin accumulation per culture was 30% and 81% higher than those in a single Phe-feeding culture and non-feeding culture, respectively. This means that the inhibitory effect of Phe on cell growth was successfully avoided and its intracellular free-state concentration was maintained at a higher level by the repetitive feeding of L-phenylalanine at relatively lower intra-medium concentration. These results support the phenylalanine pool hypothesis which states that the intracellular L-phenylalanine level determines anthocyanin production. However, it was also suggested that there exist other restrictions controlling intracellular anthocyanin accumulation.
- 公益社団法人日本生物工学会の論文
- 2005-01-25
著者
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Furusaki Shintaro
Department Of Applied Life Science Faculty Of Engineering Sojo University
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Furusaki Shintaro
Department Of Chemistry And Biotechnology School Of Engineering The University Of Tokyo:department O
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Seki Minoru
Department Of Applied Chemistry And Biotechnology Graduate School Of Engineering Chiba University
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Seki Minoru
Department Of Chemistry And Biotechnology School Of Engineering The University Of Tokyo:department O
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Nakamura Masayuki
Department Of Chemistry Faculty And Graduate School Of Sciences Kyushu University
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EDAHIRO JUN-ICHI
Department of Chemistry and Biotechnology, School of Engineering, The University of Tokyo
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Nakamura Masayuki
Department Of Chemistry And Biotechnology School Of Engineering The University Of Tokyo
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Nakamura Masayuki
Department Of Animal Science Faculty Of Agriculture Tohoku University
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Edahiro Jun-ichi
Department Of Chemistry And Biotechnology School Of Engineering The University Of Tokyo:research Cen
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Edahiro Jun-ichi
Department Of Chemistry And Biotechnology School Of Engineering The University Of Tokyo:research Cen
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