Influence of light irradiation rates and irradiation modes on caffeine production and cell growth in suspension culture of Coffea arabica cells.
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概要
- 論文の詳細を見る
The effect of light on caffeine production by <I>Coffea arabica</I> cells was investigated using chemical actinometry for the purpose of industrial application of plant cell cultures. Caffeine production by the <I>Coffea arabica</I> cells was greatly increased by light. About a six-day irradiation period was required to increase the caffeine production in a batch culture. Caffeine production significantly increased at a low light irradiation rate of 0.11 J/L/s. Meanwhile, cell growth was suppressed by increasing the irradiation rate. Maximum production was achieved at a moderate irradiation rate of 0.70 J/L/s in a 20-day batch culture. It was shown there was a light irradiation rate that optimized production. Since long-term continuous light greatly suppressed cell growth, the culture was divided into two stages: the production period in the light and the growth period in the dark. The high production and good growth were maintained for 65 days.
- The Society of Chemical Engineers, Japanの論文
著者
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Kurata Hiroyuki
Department Of Bioscience And Bioinformatics Kyushu Institute Of Technology
-
Furusaki Shintaro
Department Of Applied Life Science Faculty Of Engineering Sojo University
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Furuya Tsutomu
School Of Pharmaceutical Sciences Kitasato University
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Seki Minoru
Department Of Applied Chemistry And Biotechnology Graduate School Of Engineering Chiba University
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Furusaki Shintaro
Department of Chemical Engineering, The University of Tokyo
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