Comprehensive Phylogenetic Diversity of [FeFe]-Hydrogenase Genes in Termite Gut Microbiota
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概要
- 論文の詳細を見る
Phylogenetic diversity of [FeFe]-hydrogenase (HydA) in termite guts was assessed by pyrosequencing PCR amplicons obtained using newly designed primers. Of 8,066 reads, 776 hydA phylotypes, defined with 97% nucleotide sequence identity, were recovered from the gut homogenates of three termite species, Hodotermopsis sjoestedti, Reticulitermes speratus, and Nasutitermes takasagoensis. The phylotype coverage was 92–98%, and the majority shared only low identity with database sequences. It was estimated that 194–745 hydA phylotypes existed in the gut of each termite species. Our results demonstrate that hydA gene diversity in the termite gut microbiota is much higher than previously estimated.
- 日本微生物生態学会の論文
著者
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Xing Xin-hui
Department Of Bioengineering Tokyo Institute Of Technology
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Tanji Yasunori
Department Of Bioengineering Faculty Of Bioscience And Biotechnology Tokyo Institute Of Technology
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Zhang Chong
Department Of Chemical Engineering Tsinghua University
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Hongoh Yuichi
Department Of Biological Sciences Graduate School Of Bioscience And Biotechnology Tokyo Institute Of
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Miyanaga Kazuhiko
Department Of Bioengineering Graduate School Of Bioscience And Biotechnology Tokyo Institute Of Tech
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Bodington Dylan
Department of Biological Sciences, Graduate School of Biosciecnce and Biotechnology, Tokyo Institute of Technology
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Zheng Hao
Department of Chemical Engineering, Tsinghua University
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Tanji Yasunori
Department of Bioengineering, Graduate School of Biosciecnce and Biotechnology, Tokyo Institute of Technology
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