免疫沈降阻止反応を用いた血漿中抗てんかん薬濃度測定法の臨床への適用

概要

論文の詳細を見る
A new immuno precipitation inhibition technique (i-PiT method) was successfully applied to the determination of phenobarbital (PB) and phenytoin (PHT) in plasma. Each coefficient of variation for the 3 levels of PB or PHT was less than 10% within-run and between-run. 82 plasma specimens of epileptic patients were examined by the i-PiT method and absorptiometry. There was good agreement between the i-PiT and absorptiometry results, and the coefficients of correlation were 0.990 for PB and 0.995 for PHT. Neither primidone and carbamazepine, nor the metabolite of PB or PHT, p-hydroxyphenobarbital or p-hydroxyphenylphenylhydantoin, interfered the i-PiT method under therapeutic conditions. Therefore, the i-PiT method will be useful for the plasma level monitoring to patients undergoing PB and PHT therapy.
日本医療薬学会の論文
1981-12-20