免疫沈降阻止反応を用いた血清中カルバマゼピンとテオフィリン濃度測定法の臨床への適用

概要

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The immunoprecipitation inhibition technique (i-PiT method) was used in the clinical application to determine the concentration of carbamazepine (CBZ) and theophylline (TH) in serum. Each coefficient of variation for the 3 levels of CBZ or TH was less than 9% within・run and between run. The serum CBZ concentration determined by the i-PiT method was compared with that determined by high-performance liquid chromatography (HPLC). The correlation coefficient was 0,990, but serum CBZ concentration determined by the i-PiT method was about 15% higher than that determined by HPLC because of the cross reactivity in carbamazepine-10, 11-epoxide. Other antiepileptic drugs, such as phenytoin, Phenobarbital and primidone, did not interfere with the i-PiT method under therapeutic condition. The serum TH concentration determined by the i-PiT method was compared with that determined by HPLC. There was good agreement between the i-PiT method and HPLC results, where the correlation coefficient was 0,994. The metabolites of TH, such as caffeine and theobromine, did not interfere with the i-PiT method under therapeutic conditions. Therefore, the i-PiT method may be useful for the serum level monitoring of patients undergoing TH therapy.
日本医療薬学会の論文
1982-08-20