血清中三環系抗うつ薬濃度測定のための均一酵素免疫測定法(Emit^<[○!R]>法)の評価

概要

論文の詳細を見る
Homogeneous enzyme immunoassay method (Emit method) for the determination of tricyclic antidepressants, imipramine (IP), desipramine (DP), amitriptyline (AT) and nortriptyline (NT), in serum was evaluated. The within-run precision was determined by assaying 10 eluates of serum added antidepressants. The between-run precision was determined by assaying eluates from serum added antidepressants in 10 times during a two-week period. Each coefficient of variation value of within-run precision for each drug in serum was less than 7% and that of between-run precision was less than 9%. The serum drug concentrations by Emit method were compared with those by high-performance liquid chromatography (HPLC). There was a good correlation between HPLC and Emit method results, which correlation coefficients were more than 0.996. The cross-reactivities of six drugs in each IP, DP, AT and NT assay kit were found. No clinically significant interference was observed with hemolytic and lipetic samples at concentrations below 3g/dl hemoglobin, 400mg/dl cholesterol and 420mg/dl triglycerides. From these results, Emit method may be useful for serum drug-level monitoring of patients under the tricyclic antidepressants therapy. A lack of correlation between daily IP dose and serum IP concentration or between daily AT dose and serum AT concentration was obtained in out-patients.
日本医療薬学会の論文
1988-12-20