Purinoceptor-Mediated Calcium Mobilization and Cellular Proliferation in Cultured Bovine Corneal Endothelial Cells
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概要
- 論文の詳細を見る
In the present study, we investigated the effect of adenosine triphosphate(ATP)on cytosolic free calcium mobilization and mitogenic activity in cultured bovine corneal endothelial cells(BCEC).The[Ca2+]i was determined using a Ca2+ sensitive indicator, Fura-2/AM, and cell proliferation was evaluated by counting the cell number.ATP, its metabolites and analogs caused transient increase in[Ca2+]i in a concentration-dependent manner(10-7M−10-3M)and the potency of agonists was ordered as follows:2-methylthio-ATP>uridine triphosphate>ATP>adenosine diphosphate.Adenosine monophosphate and adenosine did not affect[Ca2+]i.ATP(10-4M)also promoted the accumulation of inositol trisphosphate(IP3).The ATP-induced transient[Ca2+]i increase and IP3 accumulation were attenuated by pretreatment with a phospholipase C inhibitor, U-73122(5μM), for 30 min.ATP(10-5M)significantly enhanced the proliferation of BCEC.ATP-induced[Ca2+]i increase and cell proliferation were inhibited by a purinoceptor antagonist, suramin(10-4M).Thus, the present study indicates that BCEC contain P2 purinoceptors that regulate their proliferation.
- 社団法人 日本薬理学会の論文
- 2000-03-01
著者
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Endou Hitoshi
Department of Pharmacology and Toxicology, Kyorin University School of Medicine
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Endou Hitoshi
群馬大学 医学系研究科病態制御内科学
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Endou Hitoshi
杏林大学 医学部薬理学教室
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Sekine Takashi
Department Of Pediatrics Gradate School Of Medicine The University Of Tokyo
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Cha Seok
Department Of Oral Physiology And The Second Stage Of Bk21 Chosun University College Of Dentistry
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Endou H
Kyorin Univ. School Of Medicine Tokyo Jpn
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LEE Kweon-Haeng
Department of Pharmacology, College of Medicine, The Catholic University of Korea
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Endou Hitoshi
Department Of Pharmacology & Toxicology Kyorin University School Of Medicine
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HAHN Tae-Won
Department of Ophthalmology, Catholic University Medical College
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Hahn T‐w
Department Of Ophthalmology Catholic University Medical College
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Sekine Takashi
Department Of Pharmacology And Toxicology Kyorin University School Of Medicine
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Lee Kweon-haeng
Department Of Pharmacology Catholic University Medical College
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Sekine Takashi
Department Of Applied Chemistry Tokyo Metropolitan University
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Hahn Tae-won
Department Of Ophthalmology Catholic University Medical College Kangnam St. Mary's Hospital
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