Inhibition of L-Type Amino Acid Transporter Modulates the Expression of Cell Cycle Regulatory Factors in KB Oral Cancer Cells
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概要
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The purpose of this study was to examine the effect of 2-aminobicyclo-(2,2,1)-heptane-2-carboxylic acid (BCH), an inhibitor of L-type amino acid transporters, on the cell growth suppression in KB human oral cancer cells and to study the roles of cell cycle regulatory factors in the BCH-induced growth inhibition. The effect of BCH on cell growth suppression and the influence of BCH to cell cycle regulatory factors in KB cell growth inhibition were examined using cell cycle analysis, immunoblotting and immunoprecipitation. The BCH treatment induced cell cycle arrest at G1 phase in KB cells. The expression of cyclin D3 was remarkably decreased by BCH treatment. The BCH inhibited the expression of cyclin-dependent protein kinase 6 (CDK6) in a time-dependent manner. In addition, the expression of CDK inhibitor p27 was increased by BCH treatment in KB cells, but not CDK inhibitors p21 and p15. These results suggest that, in KB cells, the inhibition of LAT1 by BCH causes cell cycle arrest at G1 phase by inhibiting cyclin D3–CDK6 complex whereas increasing expression of a CDK inhibitor p27.
著者
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Endou Hitoshi
Department of Pharmacology and Toxicology, Kyorin University School of Medicine
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KANAI Yoshikatsu
Department of Pharmacology and Toxicology, Kyorin University School of Medicine
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Chun Hong
Department Of Biotechnology (bk21 Program) Chosun University:research Center For Proteineous Materia
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Kim Chun
Department of Oralbiochemistry, Chosun University School of Dentistry
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Moon In-Sung
Oral Biology Research Institute, Chosun University School of Dentistry
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Park Ju-Hyun
Oral Biology Research Institute, Chosun University School of Dentistry
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Shin Woo-Cheol
Oral Biology Research Institute, Chosun University School of Dentistry
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Lee Sook-Young
Research Center for Oral Disease Regulation of the Aged, Chosun University School of Dentistry
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Kook Joong-Ki
Department of Oralbiochemistry, Chosun University School of Dentistry
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Kim Heung-Joong
Oral Biology Research Institute, Chosun University School of Dentistry
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Park Joo-Cheol
Department of Oral Histology and Developmental Biology, Seoul National University School of Dentistr
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Lee Byung-Kwon
Department of Microbiology, University of Tennessee
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Kim Do
Oral Biology Research Institute, Chosun University School of Dentistry
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Kim Heung-joong
Oral Biology Research Institute And The Second Stage Of Bk21 Chosun University College Of Dentistry
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Kanai Yoshikatsu
Department Of Pharmacol. Toxicol. Kyorin Univ.
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Endou Hitoshi
Department Of Pharmacology & Toxicology Kyorin University School Of Medicine
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Kook Joong-ki
Department Of Oral Biochemistry College Of Dentistry Chosun University
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Chun Hong
Department of Biotechnology (BK21 Program), Chosun University
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Park Joo-Cheol
Department of Oral Histology and Developmental Biology, Seoul National University School of Dentistry
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Kook Joong-Ki
Department of Biochemistry, College of Dentistry, Chosun University
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