L-Type Amino Acid Transporter-1 Expressed in Human Astrocytomas, U343MGa(Biochemistry)
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概要
- 論文の詳細を見る
LAT1 (L-type amino acid transporter l), one of the L-type amino acid transporters, transports the branched and aromatic amino acids. LAT1 requires the heavy chain of 4F2 antigen (4F2hc) for the functional expression as an amino acid transporter. The expression of this transporter is up-regulated in tumor cells and rapidly-growing cells to support their proliferation. Here, we studied the expression of LAT1 and 4F2hc in human cultured cells by real-time PCR and Western blot, and found that human brain astrocytomas, U343MGa, highly expressed LAT1 and 4F2hc mRNAs and proteins. The uptake of [^<14>C]leucine by U343MGa cells is Na^+-independent and inhibited by BCH (2-amino-2-norbornane carboxylic acid), and branched and aromatic amino acids, indicating that the LAT1 is expressed at the cell surface. Pulse chase labeling and surface labeling experiments of this cell line indicate that the protein synthesis of LAT1 and 4F2hc is slow, however, the heterodimeric complex assembled in the cells is very stable, and that the disulfide bond between the LAT1 and 4F2hc is not directly involved in the stability of the heterodimer.
- 社団法人日本薬学会の論文
- 2007-03-01
著者
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Endou Hitoshi
Department of Pharmacology and Toxicology, Kyorin University School of Medicine
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Chairoungdua Arthit
杏林大学 医学部薬理学
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Chairoungdua Arthit
Dep. Of Pharmacology And Toxicology Kyorin Univ. School Of Medicine Jpn
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Chairoungdua Arthit
Department Of Pharmacology And Toxicology Kyorin University School Of Medicine
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KANAI Yoshikatsu
Department of Pharmacology and Toxicology, Kyorin University School of Medicine
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Endou Hitoshi
群馬大学 医学系研究科病態制御内科学
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Endou Hitoshi
杏林大学 医学部薬理学教室
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TABUCHI Yoshiaki
Life Science Research Center, University of Toyama
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ASANO Shinji
Department of Molecular Physiology, College of Information Science and Engineering, Ritsumeikan Univ
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KAMEYAMA Megumi
Graduate School of Medical and Pharmaceutical Sciences, University of Toyama
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OURA Ayako
Department of Molecular Physiology, College of Information Science and Engineering, Ritsumeikan Univ
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MORISATO Anna
Department of Molecular Physiology, College of Information Science and Engineering, Ritsumeikan Univ
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SAKAI Hideki
Graduate School of Medical and Pharmaceutical Sciences, University of Toyama
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Oura Ayako
Department Of Molecular Physiology College Of Information Science And Engineering Ritsumeikan Univer
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Asano Shinji
Department Of Molecular Physiology College Of Information Science And Engineering Ritsumeikan Univer
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Morisato Anna
Department Of Molecular Physiology College Of Information Science And Engineering Ritsumeikan Univer
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Kameyama Megumi
Graduate School Of Medical And Pharmaceutical Sciences University Of Toyama
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Endou H
Kyorin Univ. School Of Medicine Tokyo Jpn
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Kanai Yoshikatsu
Department Of Pharmacology & Toxicology Kyorin University School Of Medicine
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Kanai Yoshikatsu
Department Of Pharmacol. Toxicol. Kyorin Univ.
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Endou Hitoshi
Department Of Pharmacology & Toxicology Kyorin University School Of Medicine
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Sakai Hideki
Graduate School Of Medical And Pharmaceutical Sciences University Of Toyama
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Kanai Yoshikatsu
Division Of Bio-system Pharmacology Department Of Pharmacology Osaka University Graduate School Of M
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Tabuchi Yoshiaki
Life Science Research Center Toyama Medical And Pharmaceutical University
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