Expression of Human Organic Anion Transporters in the Choroid Plexus and Their Interactions With Neurotransmitter Metabolites
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概要
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The purpose of the present study was to elucidate the expression of human organic anion transporter 1 (hOAT1) and hOAT3 in the choroid plexus of the human brain and their interactions with neurotransmitter metabolites using stable cell lines. Immunohistochemical analysis revealed that hOAT1 and hOAT3 are expressed in the cytoplasmic membrane and cytoplasm of human choroid plexus. Neurotransmitter metabolites, namely, 5-methoxyindole-3-acetic acid (5-MI-3-AA), homovanillic acid (HVA), vanilmandelic acid (VMA), 3,4-dihydroxyphenylacetic acid (DOPAC), 5-hydroxyindole-3-acetic acid (5-HI-3-AA), N-acetyl-5-hydroxytryptamine (NA-5-HTT), melatonin, 5-methoxytryptamine (5-MTT), 3,4-dihidroxymandelic acid (DHMA), 5-hydroxytryptophol, and 5-methoxytryptophol (5-MTP), but not methanephrine (MN), normethanephrine (NMN), and 3-methyltyramine (3-MT), at 2 mM, inhibited para-aminohippuric acid uptake mediated by hOAT1. On the other hand, melatonin, 5-MI-3-AA, NA-5-HTT, 5-MTT, 5-MTP, HVA, 5-HI-3-AA, VMA, DOPAC, 5-hydroxytryptophol, and MN, but not 3-MT, DHMA, and NMN, at 2 mM, inhibited estrone sulfate uptake mediated by hOAT3. Differences in the IC50 values between hOAT1 and hOAT3 were observed for DHMA, DOPAC, HVA, 5-HI-3-AA, melatonin, 5-MI-3-AA, 5-MTP, 5-MTT, and VMA. HOAT1 and hOAT3 mediated the transport of VMA but not HVA and melatonin. These results suggest that hOAT1 and hOAT3 are involved in the efflux of various neurotransmitter metabolites from the cerebrospinal fluid to the blood across the choroid plexus.
- 社団法人 日本薬理学会の論文
- 2003-12-01
著者
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Endou Hitoshi
Department of Pharmacology and Toxicology, Kyorin University School of Medicine
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Anzai Naohiko
Department of Pharmacology and Toxicology, Kyorin University School of Medicine
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Onoda Masafumi
獨協医科大学 内科学(循環器)
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Takeda Michio
Department of Pharmacology and Toxicology, Kyorin University School of Medicine, Japan
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Endou Hitoshi
群馬大学 医学系研究科病態制御内科学
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Endou Hitoshi
杏林大学 医学部薬理学教室
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Takeda Michio
Department Of Pharmacology And Toxicology Kyorin University School Of Medicine
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Endou H
Kyorin Univ. School Of Medicine Tokyo Jpn
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KHAMDANG Suparat
Department of Pharmacology and Toxicology, Kyorin University School of Medicine
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ALEBOUYEH Mahmoud
Department of Pharmacology and Toxicology, Kyorin University School of Medicine
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ONOZATO Maristela
Department of Nephrology and Endocrinology, University of Tokyo
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TOJO Akihiro
Department of Nephrology and Endocrinology, University of Tokyo
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NOSHIRO Rie
Department of Pharmacology and Toxicology, Kyorin University School of Medicine
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HASANNEJAD Habib
Department of Pharmacology and Toxicology, Kyorin University School of Medicine
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INATOMI Jun
Department of Pharmacology and Toxicology, Kyorin University School of Medicine
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NARIKAWA Shinichi
Kobuchizawa Laboratories, Fuji Biomedixs Co.
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HUANG Xiu-Lin
Kobuchizawa Laboratories, Fuji Biomedixs Co.
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Tsuda Minoru
Department Of Pharmacology And Toxicology Kyorin University School Of Medicine
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Hasannejad Habib
Department Of Pharmacology And Toxicology Kyorin University School Of Medicine
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Endou Hitoshi
Department Of Pharmacology & Toxicology Kyorin University School Of Medicine
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Endou Hitoshi
Departmentof Pharmacology And Toxicology Kyorin Universitv School Of Medicine
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Huang Xiu-lin
Kobuchizawa Laboratories Fuji Biomedixs Co.
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Inatomi Jun
Department Of Pediatrics Branch Hospital Faculty Of Medicine University Of Tokyo
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Inatomi Jun
Department Of Pharmacology And Toxicology Kyorin University School Of Medicine
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Noshiro Rie
Department Of Pharmacology And Toxicology Kyorin University School Of Medicine
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Tojo Akihiro
Division Of Nephrology And Endocrinology University Of Tokyo
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Tojo Akihiro
Department Of Nephrology And Endocrinology University Of Tokyo
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Tojo Akihiro
Department Of Internal Medicine University Of Tokyo
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Khamdang Suparat
Department Of Pharmacology And Toxicology Kyorin University School Of Medicine
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Takeda M
Pharmaceutical Laboratory Sanwa Kagaku Kenkyusho Co. Ltd
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Narikawa Shinichi
Kobuchizawa Laboratories Fuji Biomedixs Co.
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Anzai Naohiko
Department Of Pharmacology And Toxicology Kyorin University School Of Medicine
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Onozato Maristela
Department Of Nephrology And Endocrinology University Of Tokyo
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Alebouyeh Mahmoud
Department Of Pharmacology And Toxicology Kyorin University School Of Medicine
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Onozato Maristela
Department of Internal Medicine, University of Tokyo
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