Kinetic Analysis of Antibody–Antigen Interactions using Phase-Detection-Based Surface Plasmon Resonance Sensor System
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概要
- 論文の詳細を見る
Surface plasmon resonance (SPR) biosensors have the advantage of being highly sensitive, requiring no fluorescent-labeled analytes or receptors, and being capable of monitoring molecular interactions in real time. Thus, they have been widely used in determining the dynamics of molecular interactions. However, owing to the lack of experience in employing phase-detected SPR, there have been no research reports on the determination of affinity constant for antigen–antibody interactions. In this study, we used a custom-made phase-detection SPR sensor system to investigate the kinetic parameters of an antibody binding to an antigen immobilized on a CM5 sensor chip. This system provided real-time measurements of molecular interactions on the sensor chip. The association and dissociation rate constants and the corresponding affinity were obtained.
- Published by the Japan Society of Applied Physics through the Institute of Pure and Applied Physicsの論文
- 2007-05-15
著者
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Hsieh Li-zen
Department Of Electrical Engineering Chung-cheng Institute Of Technology National Defense University
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Chang Liann-be
Department Of Applied Physics Chung-cheng Institute Of Technology
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Chang Liann-Be
Department of Electronic Engineering, Chang Gung University, Taoyuan 333, Taiwan
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Joe Shen-Fen
Department of Electrical Engineering, Chung-Cheng Institute of Technology, National Defense University, Taoyuan 335, Taiwan
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Wu Chien-Ming
Department of Biomedical Engineering and Environmental Sciences, National Tsing Hua University, Hsinchu 300, Taiwan
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Hsu Chih-Ming
Department of Biomedical Engineering and Environmental Sciences, National Tsing Hua University, Hsinchu 300, Taiwan
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Hsieh Li-Zen
Department of Electrical Engineering, Chung-Cheng Institute of Technology, National Defense University, Taoyuan 335, Taiwan
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