Purification, Molecular Cloning, and Genomic Organization of Human Brain Long-Chain Acyl-CoA Hydrolase.
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概要
- 論文の詳細を見る
An acyl-CoA hydrolase, referred to as hBACH, was purified from human brain cytosol. The enzyme had a molecular mass of 100 kDa and 43-kDa subunits, and was highly active with long-chain acyl-CoAs, e. g. a maximal velocity of 295 μmol/min/mg and Km of 6.4 μM for palmitoyl-CoA. Acyl-CoAs with carbon chain lengths of C8-18 were also good substrates. In human brain cytosol, 85% of palmitoyl-CoA hydrolase activity was titrated by an anti-BACH antibody, which accounted for over 75% of the enzyme activity found in the brain tissue. The eDNA isolated for hBACH, when expressed in Escherichia coli, directed the expression of palmitoyl-CoA hydrolase activity and a 44-kDa protein immunoreactive to the anti-BACH antibody, which in turn neutralized the hydrolase activity. The hBACH eDNA encoded a 338-amino acid sequence which was 95% identical to that of a rat homolog. The hBACH gene spanned about 130 kb and comprised 9 exons, and was mapped to 1p36.2 on the cytogenetic ideogram. These findings indicate that the long-chain acyl-CoA hydrolase present in the brain is well conserved between man and the rat, suggesting a conserved role for this enzyme in the mammalian brain, and enabling genetic studies on the functional analysis of acyl-CoA hydrolase.
- 社団法人 日本生化学会の論文
著者
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Endo Takahiko
Department Of Fermentation Tachnology Faculty Of Engineering Hiroshima University
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SHIRATORI Kenji
Department of Clinical Biochemistry, Tokyo University of Pharmacy and Life Science
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Hirata Michiko
Department Of Biochemistry And Molecular Biology Tokyo University Of Pharmacy And Life Scienc : Bio-
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Yamada Junji
Department Of Clinical Biochemistry School Of Pharmacy Tokyo University Of Pharmacy And Life Science
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Taniguchi Tomomi
Department Of Clinical Biochemistry Tokyo University Of Pharmacy And Life Science
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Takama Hirotaka
Department Of Clinical Biochemistry Tokyo University Of Pharmacy And Life Science
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Kurosaki Kunihiko
Department Of Forensic Medicine Tokyo Medical University
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Kurata Akihiro
Department Of Clinical Biochemistry Tokyo University Of Pharmacy And Life Science
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Furihata Takao
Department Of Clinical Biochemistry Tokyo University Of Pharmacy And Life Science
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Shiratori Kenji
Department Of Clinical Biochemistry Tokyo University Of Pharmacy And Life Science
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Suga Tetsuya
Department Of Clinical Biochemistry Faculty Of Pharmaceutical Science Tokyo University Of Pharmacy A
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Takagi-sakuma Mitsuhiro
Department Of Clinical Biochemistry Tokyo University Of Pharmacy And Life Science
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Watanabe Takafumi
Department Of Aerospace Engineering Osaka Prefecture University
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Iida Noriko
Department Of Clinical Biochemistry Tokyo University Of Pharmacy And Life Science
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