The Cyanogenic Glucoside, Prunasin (D-Mandelonitrile-.BETA.-D-Glucoside), Is a Novel Inhibitor of DNA Polymerase .BETA..
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概要
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A DNA polymerase β (pol. β) inhibitor has been isolated independently from two organisms; a red perilla, Perilla frutescens, and a mugwort, Artemisia vulgaris. These molecules were determined by spectroscopic analyses to be the cyanogenic glucoside, D-mandelonitrile-β-D-glucoside, prunasin. The compound inhibited the activity of rat pol. β at 150 μM, but did not influence the activities of calf DNA polymerase α and plant DNA polymerases, human immunodefficiency virus type 1 reverse transcriptase, calf terminal deoxynucleotidyl transferase, or any prokaryotic DNA polymerases, or DNA and RNA metabolic enzymes examined. The compound dose-dependently inhibited pol. β activity, the IC50, value being 98 μM with poly dA/oligo dT12-18 and dTTP as the DNA template and substrate, respectively. Inhibition of pol. β by the compound was competitive with the substrate, dTTP. The inhibition was enhanced in the presence of fatty acid, and the IC50 value decreased to approximately 40 μM. In the presenceof C10-decanoic acid, the K1 value for substrate dTTP decreased by 28-fold, suggesting that the fatty acid allowed easier access of the compound to the substrate-binding site.
- 社団法人 日本生化学会の論文
著者
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Yoshida Shonen
Laboratory Of Cancer Cell Biology Research Institute For Disease Mechanism And Control Nagoya Univer
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Matsukage Akio
Laboratory Of Cell Biology
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Ogawa Akitsu
Department Of Applied Biological Science Science University Of Tokyo
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Sugawara Fumio
Department Of Agricultural Chemistry Faculty Of Agriculture Tohoku University
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Sakaguchi Kengo
Department Of Applied Biological Science Faculty Of Science And Technology Science University Of Tok
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Koshino Hiroyuki
The Institute Of Physical & Chemical Research (riken)
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Tsurugaya Kyoko
Department Of Applied Biological Science Science University Of Tokyo
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Takemura Masaharu
Laboratory Of Biology Education Graduate School Of Mathematics And Science Education Tokyo Universit
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TAKAHASHI Naoko
Department of Agriculutral Chemistry, Nagoya University
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Mizushina Yoshiyuki
Department of Applied Biological Science, Science University of Tokyo
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