遺伝子導入ショウジョウバエで過剰発現したDNAポリメラ-ゼβのDNA修復と組み換えに及ぼす影響〔英文〕

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概要

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• DNA polymerase β (pol β) cDNA of rat fused to an enhancer-promoter region plus a poly(A) signal sequence of actin 5C gene of Drosophila (abbreviated pol β) was transferred to the Drosophila genome. Three of four constructed transgenic strains possessing transgene pol β on different chromosomes were studied. Levels of the pol β transcript and those of the polymerization activity of pol β were markedly elevated in cultured cells transfected with polβ-bearing vectors as well as in embryos of the transgenic strains. The popular idea that DNA polymerase β participates in DNA repair was not supported by the observation that a pair of a normal and a polβ strain, and the other pair of a mei-9 mei-41 (DNA-repair deficient double mutations) strain and a polβ mei-9 mei-41 strain, showed no difference in survival within each pair after treatment with ultraviolet light, methylmethane sulfonate and mitomycin C. The other idea that DNA polymerase β participates in recombination was supported by the findings that spontaneous frequency of recombination, either meiotic or mitotic, is significantly higher in a transgenic polβ strain than in a non-transgenic strain. The enhanced recombination frequency in the polβ strain may, however, reflect an indirect effect of over-produced pol β proteins on chromosomal stability. Whatever the direct effect of rat pol β is, the transgenic polβ flies will be useful for study of the physiological role of pol β and the mechanism of recombination.

• 日本遺伝学会の論文
• 1994-02-25

著者

• Matsukage Akio

  Laboratory Of Cell Biology

• FUJIKAWA Kazuo

  Atomic Energy Res. Inst. Kinki Univ.

• Kondo Sohei

  Atomic E. Res. Inst. Kinki Univ.

• Fujikawa Kazuo

  Atomic Energy Research Institute Kinki University

• Nishida Yasuyoshi

  Laboratory Of Developmental Biology Division Of Biological Science Graduate School Of Science Nagoya

• Nishida Yasuyoshi

  Laboratory Of Experimental Radiology Aichi Cancer Center Research Institute

• Yamaguchi Masamitsu

  Laboratory Of Cell Biology

• Yoo Mi-ae

  Department Of Molecular Biology

• LEE Won-Ho

  Department of Biology, College of Natural Science, Pusan National University

• HA Hye-Yeong

  Department of Molecular Biology

• RYU Jae-Ryeon

  Department of Molecular Biology

• Lee Won-ho

  Department Of Biology College Of Natural Science Pusan National University

• 劉 美愛

  Department of Molecular Biology

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