Aggregation Ability of Virus-Specific Antibodies is Correlated with their Capacity to Neutralize Rice dwarf virus

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概要

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• Antibodies (immunoglobulin G (IgGs)) from antisera raised against viral particles that had been dissociated by treatment with SDS and intact particles of <I>Rice dwarf virus</I> (RDV) were studied for their ability to prevent viral infection of vector cells in monolayers<I> in vitro</I>. Even though IgGs raised against dissociated virus had a higher titer than those raised against intact viruses in an analysis of viral proteins on Western blots, they did not neutralize RDV. Conversely, IgGs raised against intact RDV effectively neutralized viral infectivity. Electron microscopic observation of the aggregation of RDV particles after incubation with IgGs raised against intact RDV, but no aggregation of RDV particles after incubation with IgGs raised against dissociated RDV suggested that IgGs raised against intact viruses might prevent viral invasion by causing clumping of viruses, thereby reducing the number of infectious units. Our results reveal, for the first time, a possible mechanism for the neutralization, mediated by antibodies, of plant viruses that propagate in insect vector cells.

著者

• Omura Toshihiro

  National Agricultural Res. Center Ibaraki

• Sakaguchi Kengo

  Department Of Applied Biological Science Faculty Of Science And Technology Science University Of Tok

• SHIMIZU Takumi

  National Agricultural Research Center, National Agriculture and Food Research Organization (NARO)

• CHEN Hongyan

  National Agricultural Research Center, National Agriculture and Food Research Organization (NARO)

• MIZUNO Hiroshi

  National Institute of Agrobiological Resources, Department of Molecular Biology

• SHIKAMOTO Yasuo

  Research Center for Stem Cell Engineering, National Institute of Advanced Industrial Science and Technology

• UEHARA-ICHIKI Tamaki

  National Agricultural Research Center, National Agriculture and Food Research Organization (NARO)

• OMURA Toshihiro

  National Agricultural Research Center, National Agriculture and Food Research Organization (NARO)

• WEI Taiyun

  National Agricultural Research Center, National Agriculture and Food Research Organization (NARO)

• SAOTOME Ai

  Department of Applied Biological Science, Tokyo University of Science

• AKITA Fusamichi

  National Agricultural Research Center, National Agriculture and Food Research Organization (NARO)

• SAKAGUCHI Kengo

  Department of Applied Biological Science, Tokyo University of Science

• MIZUNO Hiroshi

  National Institute of Agrobiological Sciences

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