Molecular Mass Measurement of Hydrophobic and Aggregative Virus Proteins to Confirm Sequence Variation and Post-translational Modification by Electrospray Ionization and Array Detection

スポンサーリンク

概要

• 論文の詳細を見る

• Molecular mass measurements have been studied of outer capsid proteins (P8, -46 kDa) of rice dwarf virus (RDV) by electrospray ionization (ESI) and array detection on a high performance magnetic sector instrument. P8 proteins were highly hydrophobic and aggregative and, therefore, hardly soluble in ESI solvents. Use of an array detector was essential to compensate for the extremely low concentration of solubilized P8 proteins. Extensive tailing of the peak profiles of multiply charged proteins made it difficult to determine their center of gravity representing relative molecular masses (Mr). In stead of Mr, top positions of unresolved peak profiles were utilized which gave molecular masses of the most abundant isotope ions (Ma) at a precision of 0.002%. Precise Ma of P8 proteins suggested the presence of an yet unknown post-translational modification, which was identified as N-terminal acetylation by the sequence analysis of V8-protease digests by FAB-MS/MS. Further, a small difference in Ma (12.3±2.1 Da) between P8 proteins from the ordinary (RDV-O) and the severe (RDV-S) strains of RDV allowed the confirmation of the strain specific amino acid substitution predicted by nucleotide sequencing. The applicability of ESI-MS in combination with array detection was demonstrated for less soluble proteins of biological importance.

• 日本質量分析学会の論文
• 1996-12-02

著者

• KOGA Yoshinori

  National Institute of Advanced Industrial Science and Technology (AIST)

• Koga Y

  National Inst. Materials And Chemical Res. Ibaraki Jpn

• OMURA Toshihiro

  National Agricultural Research Center

• ISHIKAWA Keiichiro

  National Institute of Materials and Chemical Research

• NIWA Yoshio

  National Institute of Materials and Chemical Research

• TSUCHIYA Masahiko

  Faculty of Engineering, Yokohama National University

• Omura Toshihiro

  National Agricultural Res. Center Ibaraki

• Omura Toshihiro

  National Agriculture Research Center

• Tsuchiya Masahiko

  Faculty Of Engineering Yokohama National University

• Niwa Yoshio

  National Chemical Laboratory for Industry

• ISHIKAWA Keiichiro

  National Chemical Laboratory for Industry

• Koga Yoshinori

  National Chemical Laboratory for Industry

関連論文

• Photochemical Modification of Diamond Films with Sulfur-Containing Functionalities
• 23-P-23 Tribological Properties of DLC Films on Steel Disks Deposited by Pulsed Bias CVD Method
• 23-P-20 Formation of (110) Oriented LiNbO_3 Thin Films on Polycrystalline Diamond Substrates at Low Substrate Temperature
• 23-P-19 Influence of Process Gas on the Properties of Amorphous Carbon Thin Films Deposited by Magnetron Sputtering
• 23-P-18 Chemical Modification of Diamond Powder Using Photolysis of Perfluoroazooctane
• Synthesis and Surface Acoustic Wave Property of Aluminum Nitride Thin Films Fabricated on Silicon and Diamond Substrates Using the Sputtering Method : Surfaces, Interfaces, and Films
• Binding Energies of Amorphous CN and SiCN Films on X-Ray Photoelectron Spectroscopy : Surfaces, Interfaces, and Films
• The Fraction of Sp^3 Bonding in Carbon Thin Film Prepared Using Pulsed Laser Deposition
• Formation of Cubic Phase Carbon Nitride Solid by Low Energy Nitrogen Implantation into Graphite
• Sidewall Modification of Single-walled Carbon Nanotubes with Sulfur-containing Functionalities and Gold Nanoparticle Attachment
• 23-O-06 Crystalline Carbon Nitride Film on Sapphire by Microwave Plasma CVD
• Effect of Laser Wavelength for Surface Morphology of Aluminum Nitride Thin Films by Nitrogen Radical-Assisted Pulsed Laser Deposition
• Selection of an attenuated isolate of Bean yellow mosaic virus for protection of dwarf gentian plants from viral infection in the field
• Analysis of genetic relations between Broad bean wilt virus 1 and Broad bean wilt virus 2
• Molecular Characterization of Bean yellow mosaic virus from Vegetatively Propagated Dwarf Gentiana Plants
• Preparation of Acylglucose by Invertase from Acylsucrose as a Biomimetic Surfactant
• Identification of a Polymyxin Produced by a Symbiotic Microorganism Isolated from the Brown Planthopper, Nilaparavata lugens
• 2P-020 イネ萎縮ウイルス全体構造解明を目指したP2,P7タンパク質の単離・精製・X線結晶構造解析の試み(蛋白質・構造(2),第46回日本生物物理学会年会)
• Expression and Molecular Characterization of Spherical Particles Derived from the Genome of the Hyperthermophilic Euryarchaeote Pyrococcus furiosus
• Molecular Mass Measurement of Hydrophobic and Aggregative Virus Proteins to Confirm Sequence Variation and Post-translational Modification by Electrospray Ionization and Array Detection
• In vitro but not in planta encapsidation of Rice gall dwarf virus core particles by the outer capsid P8 protein of Rice dwarf virus expressed in transgenic rice plants
• The functional organization of the internal components of Rice dwarf virus
• Stable Cluster Ions in Carboxylic Acid-Water Binary Systems and Their Reactivity with Ammonia Studied by Liquid Ionization Mass Spectrometry
• Liquid Ionizatio Mass Spectrometry for Studies on Reactions between Formaldehyde Oligomers in Aqueous Solution and Alcohols
• The Effect of Trace Iron in the Magnetic Property of the Fine Particle Prepared by ArF Laser-Induced Ablative Photodecomposition of Solid Perylenetetracarboxylic Dianhydride
• 環境プロ-ブとしての蛍光性ピレナシルペプチドリピッド〔英文〕
• Direct Dissociation of N_2O^+(X^2II_i) in the Narrow Energy Regime of 14.8-15.5 eV
• Studies on the Structures and the Fragmentations of Protonated Acetates by MINDO/3
• Effect of Various Target Molecules on a Charge Transfer Process C6H62+ → C6H6+
• Aggregation Ability of Virus-Specific Antibodies is Correlated with their Capacity to Neutralize Rice dwarf virus
• Effect of Laser Wavelength for Surface Morphology of Aluminum Nitride Thin Films by Nitrogen Radical-Assisted Pulsed Laser Deposition
• Infrared intensities in methyl chloride. III. Improvement of the anharmonic force field and the analysis of the overtone and combination band intensities.
• Infrared intensities in methyl chloride. II. Binary overtone and combination bands.
• Fragmentation of Energy-Selected Fluorobenzene Ion.
• Infrared intensities in methyl chloride. I. The fundamental bands.

もっと見る 閉じる

スポンサーリンク