Connective Tissue Growth Factor Induces the Proliferation, Migration, and Tube Formation of Vascular Endothelial Cells In Vitro, and Angiogenesis In Vivo.
スポンサーリンク
概要
- 論文の詳細を見る
Connective tissue growth factor (CTGF) is a novel cysteine-rich, secreted protein. Recently, we found that inhibition of the endogenous expression of CTGF by its antisense oligonucleotide and antisense RNA suppresses the proliferation and migration of vascular endothelial cells. In the present study, the following observations demonstrated the angiogenic function of CTGF in vitro and in vivo: (i) purified recombinant CTGF (rCTGF) promoted the adhesion, proliferation and migration of vascular endothelial cells in a dose-dependent manner under serum-free conditions, and these effects were inhibited by anti-CTGF antibodies; (ii) rCTGF markedly induced the tube formation of vascular endothelial cells, and this effect was stronger than that of basic fibroblast growth factor or vascular endothelial growth factor; (iii) application of rCTGF to the chicken chorioallantoic membrane resulted in a gross angiogenic response, and this effect was also inhibited by anti-CTGF antibodies. (iv) rCTGF injected with collagen gel into the backs of mice induced strong angiogenesis in vivo. These findings indicate that CTGF is a novel, potent angiogenesis factor which functions in multi-stages in this process.
- 社団法人 日本生化学会の論文
著者
-
NAKANISHI Tohru
Department of Biochemistiy and Molecular Dentistry, Okayama University Graduate School of Medicine a
-
TAKIGAWA Masaharu
Department of Biochemistiy and Molecular Dentistry, Okayama University Graduate School of Medicine a
-
Kuboki Takuo
Department Of Fixed Prosthodontics Okayama University Dental School
-
Shimo Tsuyoshi
Department Of Biochemistiy And Molecular Dentistry Okayama University Graduate School Of Medicine An
-
ASANO Masahiro
Department of Biochemistry and Molecular Dentistry Okayama University Dental School
-
KANYAMA Manabu
Department of Fixed Prosthodontics Okayama University Dental School
-
Matsumura Tomohiro
Deparrnfent Of Oral And Maxillofacial Surgery Ii Okayama University Dental School
-
Nishida Takashi
Department Of Biochemistry And Molecular Dentistry Okayama University Graduate School Of Medicine An
-
Takemura Motohide
Department Of Oral Anatomy And Neurobiology Osaka University Graduate School Of Dentistry
-
Tamatani Takuya
Pharmaceutical Frontier Research Laboratories Japan Tobacco Inc.
-
Tezuka Katsunari
Pharmaceutical Frontier Research Laboratories Japan Tobacco Inc.
-
Shimo Tsuyoshi
Department of Biochemistry and Molecular Dentistry, Okayama University Dental School
-
Kanyama Manabu
Department of Fixed Prosthodontics, Okayama University Dental School
関連論文
- Regulatory Mechanism of Human Connective Tissue Growth Factor (CTGF/Hcs24) Gene Expression in a Human Chondrocytic Cell Line, HCS-2/8^1
- Spectroscopic Characterization of Enamel Surfaces Irradiated with Er:YAG Laser
- Effect of 4-MET- and 10-MDP-based Primers on Resin Bonding to Titanium
- Two Mutations Convert Mammalian Xanthine Oxidoreductase to Highly Superoxide-productive Xanthine Oxidase
- REGULATION OF BIGLYCAN AND DECORIN SYNTHESIS BY CONNECTIVE TISSUE GROWTH FACTOR IN CULTURED BOVINE AORTIC ENDOTHELIAL CELLS
- Effects of repeated cyclosporin A administration on iminodipropionitrile-induced dyskinesia and TRE-/CRE-binding activities in rat brain
- Weekly 1-h paclitaxel infusion in patients with recurrent endometrial cancer : a preliminary study
- Effects of a Gonadotropin-releasing Hormone Agonist on Rat Ovarian Adenocarcinoma Cell Lines in vitro and in vivo
- Ovarian Endometrioid Adenocarcinoma Coexistent with Benign and Borderline Endometrioid Adenofibroma : A Case Report
- Anthropometric and Other Risk Factors for Ovarian Cancer in a Case-Control Study
- Transitional Cell Carcinoma of the Ovary : Report of Three Cases
- Improvement of Intraperitoneal Chemotherapy for Rat Ovarian Cancer Using Cisplatin-containing Microspheres
- Ovarian Mucinous Cystadenocarcinoma with Sarcoma-Like Mural Nodules
- Detection of Lymph Node Metastasis in Ovarian Carcinoma and Uterine Corpus Carcinoma by Preoperative Computerized Tomography or Magnetic Resonance Imaging
- Combination Treatment with Cisplatin and Schizophyllan for 7, 12-dimethylbenz(a)anthracene-Induced Rat Ovarian Adenocarcinoma
- A Case of Ovarian Serous Adenocarcinoma Suggestive of Transformation to an AFP-Producing Tumor during Chemotherapy
- HISTOLOGIC ORIGIN OF RAT OVARIAN CANCER INDUCED BY DIRECT APPLICATION OF 7,12-DIMETHYLBENZ(A)ANTHRACENE
- INTRASPLENIC GRAFTING OF 7,12-DIMETHYLBENZ (A) ANTHRACENCE-TREATED OVARIAN PIECE
- PROGNOSTIC SIGNIFICANCE OF GLIOBLASTOMA ELEMENT IN OVARIAN IMMATURE TERATOMA
- ADENOID CYSTIC CARClNOMA OF BARTHOLlN'S GLAND : A REVIEW OF THE LITERATURE AND REPORT OF A PATIENT
- A NEWER HYPERTHERMIC THERAPY WITH A BACTERIAL IMMUNOPOTENTIATOR (OK432) FOR CHEMOTHERAPY RESISTANT OVARIAN CANCER
- THERAPEUTIC EFFICACY OF CYTOTOXIC COMBINATION CHEMOTHERAPY IN OVARIAN "MATURE" TERATOMA
- DEVELOPMENT OF OVARIAN TUMORS EXPERIMENTALLY STUDIED IN MICE BY ^Co-RAY IRRADIATION
- OE-206 Disproportionate Increase of Myocardial and Plasma Connective Tissue Growth Factor against BNP is Responsible for Myocardial Fibrosis(Cardiac function, basic/clinical-1 (M) OE35,Oral Presentation (English),The 70th Anniversary Annual Scientific Mee
- Cardiac Myocytes Regulates Collagen Production Through Connective Tissue Growth Factor Secretion Induced by Various Neurohumoral Factors(Molecular Biology, Myocardium 1 (M), The 69th Annual Scientific Meeting of the Japanese Circulation Society)
- Cationic polymer-mediated genetic transduction into cultured human chondrosarcoma-derived HCS-2/8 cells
- Isolation of a mRNA Preferentially Expressed in Synoviocytes from Rheumatoid Arthritis That is Identical with Lumican, which Encodes a Collagen Binding, Extracellular Matrix Protein
- Expression of WISP-1 (ccn4), WISP-2 (ccn5) and WISP-3 (ccn6) in Rheumatoid Arthritic Synovium Evaluated by DNA Microarrays
- Expression of Tumor-rejection Antigens in Gynecologic Cancers
- Expression of the SART-1 Antigens in Uterine Cancers
- Characterization of Niobium-Doped Lead Titanate Thin Films
- Electrical Properties of LiNbO_3 Thin Films by RF Magnetron Sputtering and Bias Sputtering
- Preparation and Optical Waveguide Properties of LiNbO_3 Thin Films by IF Magnetron Sputtering
- Development of The Dentin Regeneration Therapy-Expression of Type1 collagen and ALP induced by CTGF in human cultured dental pulp-
- Development of Dentin Regeneration Therapy : Expression of Type I Collagen and Alkaline Phosphatase Induced by CTGF in Human Cultured Dental Pulp
- Theoretical Study on Surface Acoustic Wave Characteristics of LiNbO_3 Films on Sapphire Substrates
- In-plane Observations of RF-sputtered LiNbO_3 Thin Films Using an Energy Dispersive Total-Reflection X-Ray Diffractometer
- Inhibition of Neuronal Nitric Oxide Synthase by Phosphatidylinositol 4, 5-Bisphosphate and Phosphatidic Acid
- Module-Specific Antibodies against Human Connective Tissue Growth Factor : Utility for Structural and Functional Analysis of the Factor as Related to Chondrocytes
- Interaction of AP-1 and the ctgf gene : a possible driver of chondrocyte hypertrophy in growth cartilage
- Connective tissue growth factor mediates the profibrotic effects of transforming growth factor-β produced by tubular epithelial cells in response to high glucose
- Increased Levels of CTGF mRNA Expression in a Murine Model of Allergic Airway Inflammation
- Characterization and Gene Expression Profiling of Human Mesenchymal Stem Cells
- Expression of connective tissue growth factor/hypertrophic chondrocyte-specific gene product 24 (CTGF/Hcs24/CCN2) during distraction osteogenesis
- MATRICRINE AND MMPs
- Cartilaginous differentiation in the joint capsule
- CCN Family 2/Connective Tissue Growth Factor Modulates BMP Signalling as a Signal Conductor, Which Action Regulates the Proliferation and Differentiation of Chondrocytes
- Rheumatoid arthritis-related antigen 47kDa (RA-A47) is a product of colligin-2 and acts as a human HSP47
- Connective Tissur Growth Factor Iduces the Proliferation, Migration and Tube Formation of Vascular Endothelial Cells In Vitro, and Angiogenesis In Vivo^1
- Inhibition of Endogenous Expression of Connective Tissue Growth Factor by Its Antisense Oligonucleotide and Antisense RNA Suppresses Proliferation and Migration of Vascular Endothelial Cells^1
- Nitric Oxide Mediates interleukin-1-Induced Gene Expression of Matrix Metalloproteinases and Basic Fibroblast Growth Factor in Cultured Rabbit Articular Chondrocytes^1
- Specific serum antibodies against membranous proteins of a human immortal chondrocytic cell line (HCS-2/8) in rheumatoid arthritis and their relationship to the natural history of this disease
- FURTHER CHARACTERIZATION OF A HUMAN CHONDROSARCOMA-DERIVED CHONDROCYTIC CELL LINE: HCS-2/8
- Possible Roles of CTGF/Hcs24 in the Initiation and Development of Ossification of the Posterior Longitudinal Ligament
- Cyclic Mechanical Stress Induces Extracellular Matrix Degradation in Cultured Chondrocytes via Gene Expression of Matrix Metalloproteinases and Interleukin-1
- Abundant Retention and Release of Connective Tissue Growth Factor (CTGF/CCN2) by Platelets
- Processing of a Continuous Ceramic Fiber-Reinforced Iron Alloy
- Human Xanthine Oxidase Changes its Substrate Specificity to Aldehyde Oxidase Type upon Mutation of Amino Acid Residues in the Active Site : Roles of Active Site Residues in Binding and Activation of Purine Substrate
- Overexpression of CDK2 Is a Prognostic Indicator of Oral Cancer Progression
- Drainage Patterns of the Cholecystic Vein Evaluated by Power Doppler Imaging
- MALIGNANT DEGENERATION OF BENIGN CYSTIC TERATOMAS OF THE OVARY
- TREATMENT OF GYNECOLOGICAL ADENOCARCINOMAS WITH A COMBINATION OF IFOSFAMIDE, ADRIAMYCIN AND CISPLATIN
- CISPLATIN, ADRIAMYCIN AND IFOSFAMIDE-BASED COMBINATION CHEMOTHERAPY FOR GYNECOLOGICAL CANCERS DERIVED FROM THE EXTENDED MULLERIAN SYSTEM
- Atomistic Images in Field Ion Microscopy at High Temperatures
- CHEMOTHERAPY OF MALIGNANT OVARIAN TUMORS; THERAPEUTIC RESULTS OF IFOSFAMIDE
- CHARACTERIZATION OF TRANSPLANT GENERATIONS OF PRIMARY RAT ADENOCARCINOMA AND SOME CHEMOTHERAPEUTIC OBSERVATIONS
- Dopamine Receptor Presence in the Rat Area Postrema Identified by RT-PCR, Immunohistochemistry, and In Situ Hybridization
- Association of Preoperative Radiation Effect with Tumor Angiogenesis and Vascular Endothelial Growth Factor in Oral Squamous Cell Carcinoma
- Use of a porcelain laminate veneer for esthetic improvement after orthodontic treatment
- Functional MRI Study of Cerebral Cortical Activation during Volitional Swallowing
- Possible Involvement of p38 MAP Kinase in Retinoid-stimulated Expression of Indian Hedgehog in Prehypertrophic Chondrocytes
- Malignant Brenner Tumor of the Ovary : Two Cases of Unusual Histologic Features of Relapsed Tumors
- Classification System for the Completely Dentate, Partial and Complete Edentulism
- Relationship between Temporomandibular Joint Disc Position and Configuration on Magnetic Resonance Imaging
- OVARIAN TUMORS IN CHILDREN AND ADOLESCENTS LESS THAN 20 YEARS AGE
- Multiple aneurysm repair : hybrid approach to chronic dissection around visceral branches
- Mechanism of cancer-induced bone destruction : An association of connective tissue growth factor (CTGF/CCN2) in the bone metastasis
- Endovascular stent-graft repair for abdominal aortic aneurysm in a patient with cardiac and renal dysfunction
- A Carcinoma in Situ Arising in an Endocervical Polyp : A Case Report
- Effect of low-shrinking composite on the bonding effectiveness of two adhesives in occlusal Class-I cavities
- Influence of resin coating materials on Porphyromonas gingivalis attachment
- A Metastasis to the Nasal Tip from a Cervical Carcinoma. A Cace Report.:A Case Report
- Are Thyroid Neoplasms Associated with Ovarian Cancer ?
- Serous Adenocarcinofibroma of the Ovary. Report of Two Cases and Review of the Literature.:Report of Two Cases and Review of the Literature
- An experimental model for advanced ovarian cancer.
- Characterization of transplant generations of primary rat adenocarcinomas.
- A Sequential Immunosuppressive Treatment with Mizoribin (Bredinin) plus Cyclosporin A on the Subrenal Capsule Assay.
- Squamous Cell Carcinoma of the Endometrium with Human Papillomavirus Type 31.
- Minimal Deviation Adenocarcinoma (Adenoma Malignum) of the Uterine Cervix Associated with Mucinous Ovarian Carcinoma.
- Optimal Timing of a Second-Look Operation for Advanced Epithelial Ovarian Cancer.
- Experimental Intraperitoneal Administration of Cisplatin-activated Carbon for Rat Ovarian Adenocarcinomas.
- Strumal Carcinoid of the Ovary.
- Reassessment of Second Look Operation for Epithelial Ovarian Cancer.
- Experimental testicular cancer containing primarily squamous cell carcinoma. A comparative investigation of a rat ovarian cancer.:A comparative investigation of a rat ovarian cancer
- Therapeutic and staging operations for endometrial carcinomas.
- Novel pathogenic role of fibrin as revealed by a case study on ligneous gingivitis
- Inhibition of Endogenous Expression of Connective Tissue Growth Factor by Its Antisense Oligonucleotide and Antisense RNA Suppresses Proliferation and Migration of Vascular Endothelial Cells.
- Regulatory Mechanism of Human Connective Tissue Growth Factor (CTGF/Hcs24) Gene Expression in a Human Chondrocytic Cell Line, HCS-2/8.
- Connective Tissue Growth Factor Induces the Proliferation, Migration, and Tube Formation of Vascular Endothelial Cells In Vitro, and Angiogenesis In Vivo.
- Gene expression profile of mouse masseter muscle after repetitive electrical stimulation