Isolation of a mRNA Preferentially Expressed in Synoviocytes from Rheumatoid Arthritis That is Identical with Lumican, which Encodes a Collagen Binding, Extracellular Matrix Protein

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概要

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• Rheumatoid arthritis (RA) is a complex disease including autoimmune disorder and resulting in inflammation. It also shows progressive proliferation of synoviocytes, and these synoviocytes destroys articular structure. In order to understand the mechanism of this abnormal proliferation of RA-originated synoviocytes in molecular level, we analyzed the gene expression profiles by using DNA chips that contain more than 10,000 genes. Comparing the expression profiles of normal and RA-originated synoviocytes, we found several genes that are highly expressed in RA-originated synoviocytes than normal synoviocytes. Among these genes, we focused on one hypothetical protein, cDNA of which contains one reading frame in its DNA fragment, indicating that this fragment is a part of large mRNA structure. The expression of this gene in RA-originated synoviocytes is about three times higher than that in normal synoviocytes by DNA chip analysis. After cDNA cloning of this mRNA, we found that the gene is identical with lumican, which encodes a collagen binding, extracellular matrix protein. This mRNA was widely distributed in many tissues but its alternatively spliced forms are differently expressed in various tissues.

• 硬組織再生生物学会の論文

著者

• NAKANISHI Tohru

  Department of Biochemistiy and Molecular Dentistry, Okayama University Graduate School of Medicine a

• Nakanishi Tohru

  Department Of Clinical Diagnosis Shujitsu University School Of Pharmacy

• Mori Hiroki

  Department of Plastic and Reconstructive Surgery, Tokyo Medical and Dental University

• OZAKI TOSHIFUMI

  Department of Orthopaedic Surgery, Okayama University Graduate School of Medicine, Dentistry, and Ph

• Mori Hiroki

  Department Of Clinical Diagnosis Shujitsu University School Of Pharmacy

• INOUE Hajime

  Department of High Energy Astrophysics, Institute of Space and Astronautical Science, Japan Aerospac

• Ozaki Toshifumi

  Department Of Orthopaedics Okayama University Graduate School Of Medicine Dentistry And Pharmaceutic

• NISHIDA Keiichiro

  Department of Human Morphology, Okayama University Graduate School of Medicine, Dentistry, and Pharm

• Inoue H

  Department Of Orthopaedics Okayama University Graduate School Of Medicine Dentistry And Pharmaceutic

• Nishida Keiichiro

  Department Of Human Morphology Okayama University Graduate School Of Medicine Dentistry And Pharmace

• Inoue Hajime

  Department Of High Energy Astrophysics Institute Of Space And Astronautical Science Japan Aerospace

• Inoue Hajime

  Department Of Orthopaedic Surgery Faculty Of Medicine Okayama University Medical School

• Inoue Hajime

  Science Of Functional Recovery And Reconstruction Okayama University Graduate School Of Medicine And

• Nishida Keiichiro

  Department Of Orthopaedics Okayama University Graduate School Of Medicine Dentistry And Pharmaceutic

• Ozaki Toshifumi

  Department Of Orthopaedic Surgery Okayama University

• Nishida Keiichiro

  Department Of Orthopaedic Surgery Faculty Of Medicine Okayama University Medical School

• Mori Hiroki

  Department Of Applied Chemistry Graduate School Of Engineering Hiroshima University

• Inoue Hajime

  Department Of Applied Chemistry Faculty Of Engineering Kanagawa University

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