Gene expression profile of mouse masseter muscle after repetitive electrical stimulation
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概要
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Purpose: To examine gene expression profile changes in the mouse masseter muscle tissue after repetitive electrical stimulation by using a DNA microarray technique.Methods: Nine male ICR mice aged 10 weeks were used. Each anesthetized mouse was secured on a platform in a supine position and the masseter muscle tissues on both sides were exposed. Bipolar electrodes were set on the right masseteric fascia to electrically stimulate the masseter muscle (8 V, 10 Hz, 20 ms) for 30 min. After cessation of stimulation bilateral masseter muscle tissues were sampled at 0 h (n = 3),1h(n = 3), 2 h (n = 3). TotalRNAwas isolated fromthe homogenized muscle tissuesand purified mRNAsamples (50 mg) were processed and hybridized with microarray slides. Probe arrays were then scanned and analyzed to calculate the signal density. Gene expression profiles were compared at each time point between the right (stimulation side) and left (control side) masseter. When the gene expression levels were different more than 2-fold, the difference was regarded as positive.Results: Of the 6400 genes assessed, 1733 genes were up-regulated and 515 genes were down-regulated in the stimulation side at least once during the experimental time course. These up-or down-regulated genes were associated with autoimmune/inflammatory disease (28/114), cardiovascular disease (17/61), neuroscience (12/50), apoptosis (27/93), diabetes/obesity (9/28), signal transduction (66/250) and others. 28 genes were up-regulated and 25 genes were down-regulated at all time points.Conclusions: Dramatic gene expression changes were induced by the repetitive electrical muscle stimulation in mouse masseter.
- 社団法人 日本補綴歯科学会の論文
著者
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Kuboki Takuo
Department Of Fixed Prosthodontics Okayama University Dental School
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Ono Tsuyoshi
Department Of Applied Biological Chemistry Faculty Of Agriculture
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Tanaka Tsutomu
Department Of Chemical Science And Engineering Graduate School Of Engineering Kobe University
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Sonoyama Wataru
Department of Oral Rehabilitation and Regenerative Medicine, Okayama University Graduate School Medicine, Dentistry and Pharmaceutical Sciences
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Clark Glenn
Division of Diagnostic Sciences, University of Southern California School of Dentistry
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Maekawa Kenji
Department of Oral Rehabilitation and Regenerative Medicine, Okayama University Graduate School of Medicine and Dentistry and Pharmaceutical Sciences
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Kojima Shunji
Department of Oral Rehabilitation and Regenerative Medicine, Okayama University Graduate School of Medicine and Dentistry and Pharmaceutical Sciences
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