Ocular Gene Delivery Systems Using Ternary Complexes of Plasmid DNA, Polyethylenimine, and Anionic Polymers
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概要
- 論文の詳細を見る
In this experiment, we developed anionic ternary complexes for effective and safe ocular gene delivery. Ternary complexes were constructed by coating plasmid DNA (pDNA)/polyethylenimine (PEI) complex with anionic polymers such as γ-polyglutamic acid (γ-PGA) and chondroitin sulfate (CS). The cationic pDNA/PEI complex showed high gene expression on the human retinal pigment epithelial cell line, ARPE-19 cells. The pDNA/PEI complexes, however, also showed high cytotoxicity on the cells and aggregated strongly in the vitreous body. On the other hand, the anionic ternary complexes showed high gene expression on ARPE-19 cells without such cytotoxicity and aggregation. After intravitreous administration of the complexes, the anionic ternary complexes showed high gene expression in the retina. These results strongly indicate that anionic ternary complexes are suitable for effective and safe ocular gene therapy.
- 日本薬学会の論文
著者
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Uematsu Masafumi
Department of Ophthalmology and Visual Sciences, Graduate School of Biomedical Sciences, Nagasaki Un
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Sasaki Hitoshi
Department Of Basic Pharmaceutics Faculty Of Pharmaceutical Sciences Kyoto University
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Nakai Masato
Department Of Chemistry Faculty Of Science Nagoya University
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Kitahara Takashi
Department Of Breast Oncology Cancer Institute Hospital Of Japanese Foundation For Cancer Research
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Suzuma Kiyoshi
Department Of Ophthalmology And Visual Sciences Graduate School Of Biomedical Sciences Nagasaki Univ
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Kitaoka Takashi
Department of Anatomy 2, Toyama Medical and Pharmaceutical University Faculty of Medicine
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KITAOKA Takashi
Department of Ophthalmology and Visual Sciences, Graduate School of Biomedical Sciences, Nagasaki University
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Nakamura Tadahiro
Department of Hospital Pharmacy, Nagasaki University Hospital
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Kurosaki Tomoaki
Department of Drug Delivery Research, Graduate School of Pharmaceutical Sciences, Kyoto University
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Shimoda Kenichiro
Department of Hospital Pharmacy, Nagasaki University Hospital
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