Liver Site-Specific Gene Transfer Following the Administration of Naked Plasmid DNA to the Liver Surface in Mice
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概要
- 論文の詳細を見る
The present study was undertaken to investigate liver site-specific gene transfer following the administration of naked plasmid DNA (pDNA) to the liver surface in mice. We examined whether genes could be delivered to the liver site specifically by utilizing the glass-made diffusion cell that is able to limit the contact dimension between the liver surface and pDNA solution administered. Gene expression was detected at the site of diffusion cell attachment (site 1) and was significantly higher than in other liver sites and tissues. Moreover, gene expression was also detected at deeper site from the liver surface (noncontact side with pDNA solution). The level of gene expression at site 1 did not change significantly with pDNA treatment for 10, 30, and 60 min. In conclusion, we demonstrated that naked pDNA administered to the liver surface in mice was taken up from its surface, and subsequently the protein encoded by pDNA could be produced site specifically.
- 社団法人日本薬学会の論文
- 2004-10-01
著者
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中島 幹夫
佐賀大学医学部附属病院 手術部
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中島 幹夫
佐賀医科大学手術部
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中村 純三
Dep. Of Clinical Pharmacy Graduate School Of Biomedical Sciences Nagasaki Univ.
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NAKASHIMA Mikiro
Department of Hospital Pharmacy, Nagasaki University Hospital of Medicine and Dentistry
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SASAKI Hitoshi
Department of Hospital Pharmacy, Nagasaki University Hospital of Medicine and Dentistry
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Nakamura J
Faculty Of Pharmaceutical Sciences Nagasaki University
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Nakamura Junzo
長崎大学 薬
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Nakamura Junzo
School Of Pharmaceutical Sciences Nagasaki University
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Nishida K
Dep. Of Clinical Pharmacy Graduate School Of Biomedical Sciences Nagasaki Univ.
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Nakashima M
Dep. Of Clinical Pharmacy Graduate School Of Biomedical Sciences Nagasaki Univ.
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Nakashima M
Department Of Hospital Pharmacy Nagasaki University Hospital Of Medicine And Dentistry
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Nakashima Mikiro
Department Of Hospital Pharmacy Nagasaki University School Of Medicine
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Fumoto Shintaro
京都大学 薬学研究科薬品動態制御学
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Sasaki H
Dep. Of Hospital Pharmacy Nagasaki Univ. Hospital
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FUMOTO SHINTARO
Graduate School of Biomedical Sciences, Nagasaki University
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NAKAMURA JUNZO
Graduate School of Biomedical Sciences, Nagasaki University
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NISHIDA KOYO
Graduate School of Biomedical Sciences, Nagasaki University
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HIRAYAMA Ryu
Graduate School of Biomedical Sciences, Nagasaki University
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Hirayama Ryu
Graduate School Of Biomedical Sciences Nagasaki University
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Nakano M
Department Of Laboratory Medicine Nagasaki University School Of Medicine
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Sasaki Hitoshi
長崎大学 医歯学部病院薬剤部
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Sasaki Hitoshi
Department Of Basic Pharmaceutics Faculty Of Pharmaceutical Sciences Kyoto University
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Shiota H
Nagasaki Univ. Nagasaki Jpn
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Nishida Koyo
Graduate School Of Biomedical Sciences Nagasaki Univ.
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Fumoto Shintaro
Graduate School Of Biomedical Sciences Nagasaki Univ.
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Nakashima Mikiro
Department of Clinical Pharmacy, Graduate School of Biomedical Sciences, Nagasaki University
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