Stomach-Selective Gene Transfer Following the Administration of Naked Plasmid DNA onto the Gastric Serosal Surface in Mice
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概要
- 論文の詳細を見る
The purpose of the present study was to achieve a stomach-selective gene transfer following the administration of naked plasmid DNA (pDNA) onto the gastric serosal surface in mice. Gene expression in the stomach and other tissues was evaluated by firefly luciferase activity. Six hours after gastric serosal surface instillation of naked pDNA, high gene expression in the stomach was observed. On the contrary, intravenous and intraperitoneal injection of naked pDNA exhibited no detectable gene expression. Following instillation of naked pDNA onto the gastric serosal surface, gene expression in the stomach was significantly higher than in other tissues. Gene expression in the stomach was highest 12 h after the instillation and thereafter decreased gradually. Utilizing a glass-made diffusion cell that is able to limit the contact dimension between the gastric serosal surface and the naked pDNA solution administered, site-specific gene expression in the stomach was achieved. This novel gene transfer method is expected to be a safe and effective treatment against serious stomach diseases.
- 社団法人日本薬学会の論文
- 2006-10-01
著者
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中島 幹夫
佐賀大学医学部附属病院 手術部
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中島 幹夫
佐賀医科大学手術部
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中村 純三
Dep. Of Clinical Pharmacy Graduate School Of Biomedical Sciences Nagasaki Univ.
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SASAKI Hitoshi
Department of Hospital Pharmacy, Nagasaki University Hospital of Medicine and Dentistry
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Nakamura J
Faculty Of Pharmaceutical Sciences Nagasaki University
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Nakamura Junzo
長崎大学 薬
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Nakamura Junzo
School Of Pharmaceutical Sciences Nagasaki University
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Nishida K
Dep. Of Clinical Pharmacy Graduate School Of Biomedical Sciences Nagasaki Univ.
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Nakashima M
Dep. Of Clinical Pharmacy Graduate School Of Biomedical Sciences Nagasaki Univ.
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Nakashima M
Department Of Hospital Pharmacy Nagasaki University Hospital Of Medicine And Dentistry
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Fumoto Shintaro
京都大学 薬学研究科薬品動態制御学
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Sasaki H
Dep. Of Hospital Pharmacy Nagasaki Univ. Hospital
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NISHI JUNYA
Graduate School of Biomedical Sciences, Nagasaki University
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FUMOTO SHINTARO
Graduate School of Biomedical Sciences, Nagasaki University
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KODAMA YUKINOBU
Graduate School of Biomedical Sciences, Nagasaki University
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NAKASHIMA MIKIRO
Graduate School of Biomedical Sciences, Nagasaki University
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NAKAMURA JUNZO
Graduate School of Biomedical Sciences, Nagasaki University
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NISHIDA KOYO
Graduate School of Biomedical Sciences, Nagasaki University
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SHOJI Keiko
Graduate School of Biomedical Sciences, Nagasaki University
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Shoji Keiko
Graduate School Of Biomedical Sciences Nagasaki University
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Kodama Yukinobu
Graduate School Of Biomedical Sciences Nagasaki Univ.
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Nakano M
Department Of Laboratory Medicine Nagasaki University School Of Medicine
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Sasaki Hitoshi
長崎大学 医歯学部病院薬剤部
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Sasaki Hitoshi
Department Of Basic Pharmaceutics Faculty Of Pharmaceutical Sciences Kyoto University
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Shiota H
Nagasaki Univ. Nagasaki Jpn
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Sasaki Hajime
Semiconductor Group Mitsubishi Electric Corporation
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Sasaki Hideyuki
R&d Center Toshiba Corp.
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佐々木 均
Department of Hospital Pharmacy, Nagasaki University School of Medicine
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Sasaki H
Central Research Laboratories Zeria Pharmaceutical Co. Ltd.
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Sasaki H
Electrotechnical Laboratory
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Sasaki H
Nagasaki Univ. School Of Medicine Nagasaki Jpn
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Nishida Koyo
Graduate School Of Biomedical Sciences Nagasaki Univ.
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Sasaki Hitoshi
Department Of Hospital Pharmacy Nagasaki University Hospital
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中村 純三
Graduate School Of Biomedical Sciences Nagasaki Univ.
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Fumoto Shintaro
Graduate School Of Biomedical Sciences Nagasaki Univ.
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麓 伸太郎
Graduate School of Biomedical Sciences, Nagasaki University
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土持 真由美
Graduate School of Biomedical Sciences, Nagasaki University
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兒玉 幸修
Graduate School of Biomedical Sciences, Nagasaki University
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西 順也
Graduate School of Biomedical Sciences, Nagasaki University
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中嶋 幹郎
Graduate School of Biomedical Sciences, Nagasaki University
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西田 孝洋
Graduate School of Biomedical Sciences, Nagasaki University
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