Electrophysiological Characterization of Tight Junctional Pathway of Rabbit Cornea Treated with Ophthalmic Ingredients
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概要
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The purpose of this study was to investigate the continuous and real-time influence of ophthalmic ingredients on rabbit cornea by monitoring electrophysiological characteristics. The tight junctional permeabilities of FITC-dextran 4400 (FD-4) was also determined through the cornea in the presence of ophthalmic ingredients. Intact cornea showed approximately one k-ohm×cm2 of transepithelial electrical resistance (TEER) and extremely low permeability of FD-4. The ophthalmic ingredients used in the present study were benzalkonium chloride (BK; 0.002%, 0.01%, 0.05%), ethylenediaminetetraacetic acid (EDTA; 0.5%), capric acid (C10; 0.25%), saponin (SP; 0.1%), taurocholic acid (TA; 1.0%) and sodium dodecyl sulfate (SDS; 0.01%). They were previously reported to be effective on corneal penetrations of various drugs at those concentrations without severe toxicity. These ingredients decreased TEER and increased corneal permeability of FD-4. BK reduced TEER in a concentration-dependent manner. There was a significant correlation (γ=0.860) between the permeability coefficient (Papp) of FD-4 and conductance (Gm), which is the reciprocal value of TEER. It was also indicated that Papp and Gm have a relationship with the corneal cytotoxicity of the ingredients. In conclusion, an electrophysiological method using isolated cornea was very useful to determine the continuous and real-time influence of ophthalmic ingredients on the cornea. In this method, electrophysiological conductance must be able to predict corneal tight junction permeability and the corneal cytotoxicity of ingredients.
- 社団法人日本薬学会の論文
- 2007-12-01
著者
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中島 幹夫
佐賀大学医学部附属病院 手術部
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中島 幹夫
佐賀医科大学手術部
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NAKASHIMA Mikiro
Department of Hospital Pharmacy, Nagasaki University Hospital of Medicine and Dentistry
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SASAKI Hitoshi
Department of Hospital Pharmacy, Nagasaki University Hospital of Medicine and Dentistry
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Nakashima M
Dep. Of Clinical Pharmacy Graduate School Of Biomedical Sciences Nagasaki Univ.
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Nakashima M
Department Of Hospital Pharmacy Nagasaki University Hospital Of Medicine And Dentistry
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Nakashima Mikiro
Department Of Hospital Pharmacy Nagasaki University School Of Medicine
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Horimoto Tomomi
Department Of Hospital Pharmacy Nagasaki University Hospital Of Medicine And Dentistry
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To Hideto
長崎大学 医歯学部病院薬剤部
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To Hideto
Department Of Hospital Pharmacy Nagasaki University Hospital Of Medicine And Dentistry
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Sasaki H
Dep. Of Hospital Pharmacy Nagasaki Univ. Hospital
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ICHIKAWA Nobuhiro
Department of Hospital Pharmacy, Nagasaki University Hospital of Medicine and Dentistry
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NAKAMURA Tadahiro
Department of Hospital Pharmacy, Nagasaki University Hospital of Medicine and Dentistry
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YAMADA Mikiko
Department of Clinical Pharmacy, Graduate School of Biomedical Sciences, Nagasaki University
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TESHIMA Mugen
Department of Hospital Pharmacy, Nagasaki University Hospital of Medicine and Dentistry
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Ichikawa Nobuhiro
Department Of Hospital Pharmacy Nagasaki University Hospital Of Medicine And Dentistry
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Nakano M
Department Of Laboratory Medicine Nagasaki University School Of Medicine
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Yamada Mikiko
Department Of Clinical Pharmacy Graduate School Of Biomedical Sciences Nagasaki University
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Sasaki Hitoshi
長崎大学 医歯学部病院薬剤部
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Sasaki Hitoshi
Department Of Basic Pharmaceutics Faculty Of Pharmaceutical Sciences Kyoto University
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Shiota H
Nagasaki Univ. Nagasaki Jpn
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Sasaki Hajime
Semiconductor Group Mitsubishi Electric Corporation
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Sasaki Hideyuki
R&d Center Toshiba Corp.
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Sasaki H
Central Research Laboratories Zeria Pharmaceutical Co. Ltd.
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Sasaki H
Electrotechnical Laboratory
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Sasaki H
Nagasaki Univ. School Of Medicine Nagasaki Jpn
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Sasaki Hitoshi
Department Of Hospital Pharmacy Nagasaki University Hospital
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To Hideto
Department Of Hospital Pharmacy Nagasaki University Hospital
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Nakashima Mikiro
Department of Clinical Pharmacy, Graduate School of Biomedical Sciences, Nagasaki University
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Nakamura Tadahiro
Department of Hospital Pharmacy, Nagasaki University Hospital
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