Assay of Indoleacetic Acid in Semm by a GC-MS Spectmmeter

概要

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lndolacetic acid is one of the metabolites of tryptophan, As its levels in body fluid is very Iow, it has been difficult to estimate it by conventional analytical methods.<BR>In the present study, an attempt was made to employ gas Chromatogram/mass spectrometry (GC/MS) for the determination of the Ievels in serum from patients with several disease, such as gastric and cobn cancer, hepatoma and other gastroenteric diseasee.<BR>For GC/MS was used Finnigan Quardrupole Type Mass Spectrometer. Quantitative analyses of indolaceticacid in serum were performed by the multiple ion detectation technique, because its sensitivity was higher than the total ion monitoring technique, and indolcarbomc acid was used as an internal standard, which is absent in natural organisms.<BR>Possible usefulness for diagnostic examination of cancers was also discussed.
Japan Society of Clinical Chemistryの論文