A Measure to Counter Interference of the Ketonuria Test by Cephems

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The stick methods, utilizig Sodium Nitroprusside, are generally used for the monitoring of ketonuria in clinical laboratory tests.The influence of Cephacetrile and various other Cephem series of antibiotics on this test was studied. Cephacetrile iterfered with the ketonuria test to produce reddish orange color differig from the purple Color of ketoacidosis when ketone body's absent in the urine. Other Cephems almost always gave a dark orange color with the passage of time in the same way. This kind of coloration may lead to incorrect readigs of ketonuria when the test is applied to urine sample containing Cephems or its degradation, metabolites. Fresh urine should therefore be used in the test.Other countermeasures are needed when testing urie in patients receiving Cephacetrile and in cases where fresh urine samples are difficult to obtain and when it is difficult to read differences in the shades of dark orange due to the inexperience of the tester. ln present study, we established a simple and useful method against this iterference. The method involves the acidification of the urie sample with N HCl and treatment with a small activated charcoal column, and war tried in the determination of ketone bodies in urine obtaied from healthy volunteers and in patients receiving Cephems. Satisfactory rerults without any iterference were obtained.
Japan Society of Clinical Chemistryの論文