Investigation of Pre-Incubation Time on Creatine Kinase Activity Assay

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The members of German Society for Clinical Chemistry (GSCC) committee contributed considerably to standardization of the enzyme activity assay and a reference method for creatine kinase (EC 2.7.3.2, CK) activity assay in which coupling enzyme reaction with hexokinase (EC 2.7.1.1) and glucose-6-phosphate dehydrogenase (EC 1.1.1.49) is used has been proposed in 1977.<BR>This reference method for CK activity assay by GSCC seems to be a superior method because of the choice of activator for CK and avoidance of interfering reaction by adenylate kinase.<BR>Reagent kit for CK activity assay (Boehringer-Mannheim Corp.) which composition is based on the reference method of GSCC is used for evaluation of the CK activity assay.<BR>We obtained different result in point of a pre-incubation time, that is, according to the reference method of GSCC, pre-incubation time required is 5 min at 25° because significant difference was not recognized with pre-incubation of 5, 10, 20, 30, 60, 120, and 360min.<BR>But our results showed that 30 min of pre-incubation is required to reach maximum activity for a serum sample, and about 60min for some control serum sample
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