Enzymatic Determination of Serum Uric Acid:Using Uricase and NADH-Peroxidase
スポンサーリンク
概要
- 論文の詳細を見る
Enzymatic determination of serum uric acid using uricase (EC. 1. 7. 3. 3) and NADH-peroxidase (EC. 1. 11. 1. 1.) is described.<BR>The principle of this method is as follows: Uric acid is degradated by uricase to produce allantoin and H<SUB>2</SUB>O<SUB>2</SUB>, and the H<SUB>2</SUB>O<SUB>2</SUB> produced is coupled with NADH-peroxidase. The change in absorbance at 340 nm is measured. Standard curve was linear up to, at least, 20mg/dl in concentration. Correlation between present method and UV method (assay at 291 nm) was compared, and the correlation coefficent value was 0.998 and regression line was y=1.00x-0.08 (y: present method).
- Japan Society of Clinical Chemistryの論文
著者
-
林 長蔵
大阪大学医学部附属病院中央臨床検査部
-
古川 一郎
大阪大学医学部附属病院中央臨床検査部
-
古川 一郎
大阪大学医学部付属病院中央臨床検査部
-
中西 千恵子
大阪大学医学部附属病院中央臨床検査部
-
林 長蔵
大阪大学医学医学部附属病院中検臨床検査部
関連論文
- Disequilibrium syndrome に関する臨床的研究
- 脂肪酸活性化酵素反応を用いた脂肪酸定量へのアプローチ
- The Rapid and Simple Determination of Urinary 3-Methoxy-4-Hydroxymandelic Acid Eliminated the Interference of Exogenous Vanillin
- Kinetic Studies on Determination of Serum Cholineesterase Activity Using Choline Oxidase
- A Measure to Counter Interference of the Ketonuria Test by Cephems
- Determination of Serum Pyruvate Concentration Using Enzymatic Cycling
- Interference of Cefoxitin on Urinary 17-OHCS Assay
- Application of Electron Spin Resonance (ESR) to Determination of Total Protein in Cerebrospinal Fluid
- Regulation of Serum Cholesterol Level by the Liver:Relationship with Development of Hypercholesterolemia, Aging and Genetic Factor
- Interference of peptide binding antibiotics in urinary steroid hormone assay
- Evaluation of Guanidino Compounds Values using Samples Drown with Glass and Polyethylene Syringe and the Comparison of Values from Serum and Plasma
- Enzymatic Method Assay of Inulin in Human Serum and Urine
- Automated Estimation of HDL-cholesterol by Continuous Ultrafiltration System.
- Determination of Guanidino Compounds by High-Speed Liquid Chromatography and its Clinical Significance
- Investigation of Pre-Incubation Time on Creatine Kinase Activity Assay
- A Kinetic Method for Measurement of Adenosine Deaminase Isozymes Using Different Concentrations of Substrate
- Determination of Guanidino Compounds by HPLC
- Iodinations of Cholecystokinin and Human Parathyroid Hormone for Radioimmunoassay
- Clinical Application of Hormone Enzyme Immunoassay
- Studies on Constituents in Human Serum (1):Analysis of Cholesterol and Cholesterol Ester
- Human serum transaminases: Fundamental studies on assay methods based on enzyme properties
- High Sensitive Fluorometric Determination of Bile Acids in Biological Fluid with High Performance Liquid Chromatography and Immobilized Enzyme
- Abnormal Result of Clinical Test with Cephacetrile and An Immediate Counterplan
- The Analysis of Prostaglandins by High Performance Liquid Chromatography Using Pre Column Derivatization with 9-Anthryldiazomethane
- A Procedure for the Detection of Alkaline Phosphatase Isoenzyme using 4-Methylumbelliferyl Phosphate as a Substrate
- Assay of Indoleacetic Acid in Semm by a GC-MS Spectmmeter
- Enzymatic Determination of Serum Uric Acid:Using Uricase and NADH-Peroxidase
- Studies of α-HBDH Activity Assay;Effect of Incubation Temperature on α-HBDH Activity.
- Studies on Constituents in Human Serum (2) Analysis of Grycerides
- A NEW INSTRUMENT FOR BILIRUBIN MEASUREMENT
- Determination of Guanidino Compounds by High Performance Liquid Chromatography
- Evaluation of E3 kit; Interference of Protein and Some Antibiotics.
- To continuous flow system
- Investigation of Mechanism for Ammonia Production with Standing after Venipuncture
- Automatic Kinetic Enzyme and Substrate System (AKES Vitatron)