Iodinations of Cholecystokinin and Human Parathyroid Hormone for Radioimmunoassay
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概要
- 論文の詳細を見る
For cholecystokinin (CCK)-specific radioimmuoassay (RIA), CCK-33 and CCK-8 were iodinated by <SUP>125</SUP>1-Bolton-Hunter (BH) reagent. Both RIAs using <SUP>125</SUP>I-BH-CCK-33 and <SUP>125</SUP>1-BHCCK-8 were identical in sensitivity and specificity. On the other hand, the RIA using <SUP>125</SUP>I-BHCCK-33 allowed us to measure CCK in plasma without prior extraction of plasma samples, while extraction of plasma was required for RIA using <SUP>125</SUP>I-BH-CCK-8. Histidine residues of synthetic human parathyroid (hPTH) 1-34 were iodinated by chloramine T for N-terminal PTH RIA. The specific radioactivity of the <SUP>125</SUP>I-hPTH1-34 was 110μCi/μg and immunological activity was almost maintained by the iodination.
- Japan Society of Clinical Chemistryの論文
著者
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林 長蔵
大阪大学医学部付属病院中央臨床検査部
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立石 カヨ子
大阪大学医学部附属病院中央臨床検査部
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浜岡 利之
大阪大学医学部癌研
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橋村 悦朗
大塚アッセイ研究所
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立石 カヨ子
大阪大学医学部付属病院中央臨床検査部
-
立石 カヨ子
大阪大学医学部中央臨床検査部
-
林 長蔵
大阪大学医学医学部附属病院中検臨床検査部
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