Enzymatic Method Assay of Inulin in Human Serum and Urine

概要

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An enzymatic assay method using inulase and sorbitol dehydrogenase (SDH) for determination of inulin in human serum and urine was described.<BR>0.2ml of serum or 100 times diluted urine was mixed with lml of O.2M acetate buffer of pH5.Oand 20μl of inulase solution.Following incubation of the mixture for 60min.at 37°, 0.2ml of 1.0M triethanolamine buffer of pH8.2, 20μl of NADH soution and 20μl of LDH solution were added.<BR>The mixture was then incubated further at 37° for ca.10min.lmmediately after addition of SDH solution (50μl), reduction of absorbance was measured at 340nm.<BR>The linear relationship was observed between the amount of inulin and the absorbance.The average recovery of inulin added to the serum and urine were 98.7%and 100.2% respectively.The reproducibility of the me thod was 3.4% (C.V.) with 50.1±1.70mg/dl (mean±S.D.).Giucose present in the sample was found to be inert in the present method.<BR>The results obtained by the present method corresponded with those obtained by the modified Heyrovsky's method.
Japan Society of Clinical Chemistryの論文