Direct Analysis Method for Urinary Catecholamines by High Performance Liquid Chromatography and its Clinical Application

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Using the high performance liquid chromatography, a quantitative, specific, and direct method for urinary catecholamines [adrenaline (Adr.), noradrenaline (NA) and dopamine (DA)] was established. Principle of our present method is that with high performance liquid chromatograph, catecholamines were selectively separated from other urinary components by gradient elution method. For continuous detection of catecholamine fractions trihydroxyindole procedure was employed. Each catecholamine was separated definitely from other urinary components. With this method we tried to standardize some of problems concerning pretreatment of urine for analysis of catecholamines. The present results showed that pH≤53.0,?≤4°C were suitable for storage and that pH≤1.0, 100?°C, 30 min. for hydrolysis of the urine. In comparison of the determined values of urinary catecholamines with the clinical courses of the patients, a correction to devide determined values by glomerular filtration rate was found to be useful for clinical application. Using this method of the correction, prognosis of patients with brain aneurysm under surgical procedure has been studied and following results were obtained: catecholamine values (corrected) were found much higher (over 1,000pg/ml) in patients with aneurysm of unfavourable prognosis than those of good or in patients under surgery of unilateral carotid artery occlusion as controls. On the basis of our present observation, it might be suggested that urinary catecholamines were available as a parameter to evaluate prognosis of patients with brain aneurysm under surgical procedure.

Direct Analysis Method for Urinary Catecholamines by High Performance Liquid Chromatography and its Clinical Application

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