Direct Analysis Method for Urinary Catecholamines by High-performance Liquid Chromatography

概要

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Using high performance liquid chromatography, a quantitative, specific and direct determination method for urinary catecholamines [Adrenaline (Adr.), Noradrenaline (NA) and Dopamine (DA)] was established. The principle of our present method is that with high-performance liquid chromatograph, catecholamines were selectively separated from other urinary components by gradient elution method. For continuous detection of catecholamine fractions, trihydroxyindol procedure was employed. Each catecholamine was separated definitely from other urinary components. Only 60 minutes was required for analysis of one sample. As little as 10-20μl urine specimens were found to be enough for the analysis. The sensitivity was 1.0ng/ml for Adr., 1.5ng/ml for NA and 150ng/ml for DA. The coefficient of variation byintra-assay (n=5) was ±2.9% for Adr.,±3.0% for NA and ±5.0% for DA. The coefficient of variation by inter-assay (n=7) was±1.7% for Adr.,±2.3% for NA and ±7.2% for DA. The average of recovery was 101.4% for Adr., 98.7% for NA and 97.7% for DA. Thus, the present methodhas proved to be feasible for the specific assay of urinary catecholamines in monitoring patients with hypertention.
Japan Society of Clinical Chemistryの論文