The Analysis of Prostaglandins by High Performance Liquid Chromatography Using Pre Column Derivatization with 9-Anthryldiazomethane

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概要

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• The analysis of prostaglandins (PGs) were investigated by high performance liquid chromatography (HPLC) using 9-anthryldiazomethane (ADAM).<BR>ADAM, which is the labeling reagent of carboxylic group, can react with PGs rapidly. The derivatives were separated in reversed phase chromatography using Zorbax ODS column (4.6mm i. d.×25cm). Methanol-water was found to be suitable for complete separation of ADAM esters of PGE<SUB>1</SUB>, PGE<SUB>2</SUB>, PGF<SUB>1α</SUB> and PGF<SUB>2α</SUB> within 30 minutes.<BR>The PGs-ADAM esters can be detected by fluorophotometer at Ex. 365nm Em. 412nm.<BR>In this method, the fluorescence intensity of the derivatives have wide linearity over 100ng and detection limit was 100pg.

• Japan Society of Clinical Chemistryの論文

著者

• 林 守正

  株式会社島津製作所分析計測事業部lcビジネスユニット

• 有末 一隆

  大阪大学医学部付属病院中央臨床検査部

• 林 長蔵

  大阪大学医学部付属病院中央臨床検査部

• 甲田 一馬

  大阪大学医学医学部附属病院中検臨床検査部

• 甲田 一馬

  大阪大学医学部付属病院中央検査部

• 石田 泰夫

  株式会社島津製作所第一科学計測事業部

• 植田 季弘

  株式会社島津製作所分析センター

• 林 長蔵

  大阪大学医学医学部附属病院中検臨床検査部

• 有末 一隆

  大阪大学医学医学部附属病院中検臨床検査部

• 有末 一隆

  大阪大学医学部付属病院中央検査部

• 石田 泰夫

  株式会社島津製作所

• 林 守正

  株式会社島津製作所分析センター

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