Determination of Human Urinary Kallikrein by an Enzyme Immunoassay Method

概要

論文の詳細を見る
An enzyme immunoassay (EIA) method for determination of human urinary kallikrein has been established. At least 5ng of kallikrein per ml urine could be determined, and reliabilty of this method was confirmed by the reproducibility, the dilution test and the recovery test. Several properties of the urinary kallikrein were shown by this method. When the urine was treated with trypsin, the amount of kallikrein increased by about 2-to 5-fold. With both control and the trypsin-treated urine, a linear relationship was obtained between the amount of kallikrein and kallikrein activity, and the slope of the line with control urine was less than that with the trypsin-treated urine. Kallikrein, prokallikrein and trypsin-activated prokallikrein formed a smoothly fused precipitin line by the reaction with rabbit antihuman urinary kallikrein antiserum in Ouchterlony's double diffusion experiment. These results indicate that only the active form of kallikrein is detectable by the EIA method, although both kallikrein and prokallikrein are reactive toward the anti-kallikrein antiserum. It is also indicated that about 50-75% of kallikrein exists as prokallikrein in the urine.
Japan Society of Clinical Chemistryの論文