A Modified Automated Simultaneous Determination of Uric Acid and Creatinine
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This report presents a newly-modified procedure for the automated simultaneous determination of uric acid and creatin ine in serum. Uric acid was assayed by photometrical measurements of colour produced with neocuproine. Neocuproine (less soluble in water) was dissolved in ethanol, and ethylene glycol was added to it (23.3vol%) so as to fit for an Auto-Analyzer analysis by increasing stability and viscosity. Jaffe's method with picric acid was applied to the determination of serum creatinine.<BR>Among many reducing substances in Vivo, which were presumed to influence determinations, ascorbic acid and glutathione was shown to be a cause for notably higher-values.<BR>Establishment of normal range of serum uric acid values was tried on 104healthy persons, as there had been noreliable reports on it. A slightly higher value(5.4±2.5mg/dl) was obtained than other several reports, especially on the male.<BR>Coefficients of variation of this modified methods were±5%for uric acid and±3%for creatinine. These methods correlated highly to usual manual method (r=0.99respectively).
- Japan Society of Clinical Chemistryの論文
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