MacroprolactinomaにおけるhPRLのHeterogeneityの検討

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The heterogeneity of hPRL in the serum of a patient with a giant prolactinoma (hPRL 27 μg/ml) was studied employing gel chromatography, RIA and lymphoma cell bioassay. After gel filtration of serum sample on Sephadex G-100 (superfine) column, a few hPRL peaks were detected by RIA. Rechromatography of the second peak, which eluted between the void volume and the small third peak, revealed four hPRL peaks designated big-big hPRL, medium-big hPRL, big hPRL and little hPRL, with BA/RIA ratio 1.3-2.6, 0.8-1.2, 0.3-0.7 and 0.7-1.5, respectively. The molecular weight of medium-big hPRL was estimated as 80K-90K daltons. Most of big-big, medium-big and big hPRL were converted to little hPRL after mercaptoethanol treatment. These data suggest that most of big-big, medium-big and big hPRL are aggregates of little hPRL.
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