p53癌抑制遺伝子関連抗原と増殖細胞核抗原 (PCNA) の免疫組織学的発現とDNA aneuploidy との関連性について

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The immunohistochemical staining of p53 was detected not only in squamous cell carcinoma but also in normal stratified epithelium. The immunostaining of p53 and PCNA was localized in similar areas of cancer tissue. The number of positive cells was counted using image analysis system PIAS LA 555. PCNA and p53 positive rates were 74.0%(108/146) and 22.6%(33/146), respectively. PCNA and p53 scores were 34.6 ± 2.4%(n=108) and 26.8 ± 4. 1%(n=33) as mean ± SE. Flow cytometrical analysis revealed 20 cases of diploidy and 6 cases of aneuploidy among 146 cases of squamous cell carcinomas. PCNA score was 49.9 ± 5.2%(n=16) in diploidy and 37.5 ± 13.8%(n=5) in aneuploidy. p 53 score was 35.6 ± 12.6%(n=5) in diploidy, and 25.9 ± 16.5%(n=2) in aneuploidy. These results suggested similarities in immunostaining between p53 and PCNA, and that p 53 score was smaller than PCNA score. Further investigation is required to clarify the relationship between p53 and DNA ploidy pattern.
社団法人日本口腔外科学会の論文