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A new method of radioimmunoassay using double antibody method for human group II pepsinogen (PG II) in serum was developed. 1) In this system, optimal antibody concentration is 2, 000 times dilution of original antiserum. sensitivity of the assay is in the order of 1 ng.per ml of serum and optimal assay range is 10 to 50 ng per ml. Enzymatic activity of 1 ng human PG II is 19.6 units under the condition of pH 2.0 as determined by the method of Anson with a slight modification. 2) No effect of interference of human serum is detected in this system and B/T value of 500 ng per ml of purified human PG I is almost equal to that of 1 ng per ml of purified human PG II. With these results, it is quite permissible to say that only the substances in serum which are immunochemically analogous to human PG II are detected by the system. 3) satisfactory results were obtained as for both intra and between assay reproducibility (coefficient of variation was 3.9% and 3.7-15.3% respectively). 4) The mean (±s. E. ) serum PG II levels in healthy donars were 16.0±0.8 ng/ml for males and 12.4±1.4 ng/ml for females. The difference between males and females was statistically significant (P<0.05). 5) The mean (±s. E. ) serum PG II level of 4 patients with total gastrectomy was 1.1±0.07 ng/ml. These results suggest that most of PG II in human serum is derived from gastroduodenal system.
財団法人日本消化器病学会の論文