Studies on alkaline phosphatase isoenzymes in carcinoma tissue of the stomach

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Alkaline phosphatase (ALP) isoenzymes in carcinoma tissues of the stomach were investigated on their enzymological and immunological properties.Twenty three specimens of the gastric carcinoma tissues were obtained removed at operation. Their ALPs were extracted with n-butanol by a modification of Morton's method. These were separated into 3 bands of ALP isoenzymes (ALPa, ALPb and ALPc) by polyacrylamide-gel disc electrophoresis. It was•concluded that the enzymological and immunological properties of ALPa was similar to those of hepatic ALP, ALPb similar to placental ALP and ALPc to intestinal ALP.ALPb, which appeared in 8 out of 23 cases (35%) of gastric carcinoma tissues, showed heat-stability at 65°C for 20min. incubation, and seemed to be originated from the cancer cells themselves. ALPc, which appeared in 9 cases (39%), and the other 14 cases (61%) had not intestinal ALP, although various intensities of surrounding intestinal metaplasia were found in 21 cases (91%), and seemed to be originated from surrounding intestinal metaplasia. ALPa detectable in carcinoma tissue extract, which appeared in all cases and its enzymological and immunological properties were similar to normal mesenchymal cells, seemed not to be originated from the cancer cells but from the tissues surrounding cancer cells. There were no relationships between the above 3 isoenzymes and histological findings or Borrmann's macroscopic classifications. But, in well-differentiated type of gastric cancer, the intestinal metaplasia appeared more intensively than in poorly-differentiated type, and we could not deny completely the possibilities of producing the intestinal and hepatic ALPs from the cancer cells.
財団法人日本消化器病学会の論文

Studies on alkaline phosphatase isoenzymes in carcinoma tissue of the stomach

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