:Using the Methods of Succinylated Albumin as Substrate

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Pepsinogen (Pg) activity of gastro-duodenal mucosa were determined using biopsy specimens in 95 cases, both in-and-out patients of our department (Mean age 52 y.o., Gastric cancer 27, Gastric ulcer 26, Duodenal ulcer 12, Normal & others 21). That is, biopsy samples were collected at times of endoscopical examination in these subjects from duodenum, antrum, angle and corpus under direct observation. Two samples from each part, one for Pg assay and the other for histological evaluation such as the existence of intestinal metaplasia and type of gastric gland. From these collected 355 samples in a series of 100 endoscopical examination, Pg was extracted by centrifugation at 105, 000xg for 60 min at 4°C and supernatant was used for assay. Pg activity was measured by the method using succinylated albumin as substrate, and protein concentration, by the method of modification of Lowry's.From these aquired Pg activity per mg protein, following results were obtained: 1) Mucosal Pg activity gradually gets higher from pylorus to corpus (P<0.001). 2) Corelation between histology of gastric glands and Pg activity was, func gland>intermediate gland>pylolic gland (P<0.001). 3) The difference of Pg activity between gastric mucosa with and without intestinal metaplasia was statistically significant (P<0.01). 4) In relation to various diseases, mucosal Pg activity of duodenal ulcer was significantly higher than normal and gastric ulcer (P<0.001) in both gastric and duodenal mucosa.
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:Using the Methods of Succinylated Albumin as Substrate

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