Quenching effects of various compounds on fluorometric assay of tryptophan.
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概要
- 論文の詳細を見る
Quenching effects of 26 kinds of compounds on the fluorometric assay of tryptophan were studied. Investigated compounds were monoamines, their precursors and metabolites, and drugs used in the study of monoamines. The fluorescence of 1μg of tryptophan was decreased in the presence of the same amount of L-DOPA, catecholamines, DOPAC, 5-HTP, 5-HT, MK 486 or Ro 4-4602 when these samples were prepared in 0.1 M phosphate buffer (pH 7.4) and estimated by the method of Denckla and Dewey. The degree of quenching was varied according to the diluent of the compounds and tryptophan. These results suggest that the direct application of the ultraf iltrate for the assay of free tryptophan might be quenched after administration of drugs. The decreased fluorescence of tryptophan by above compounds, except 5-HTP, was recovered after introducing separation procedure of tryptophan by the Dowex column.
- 久留米大学医学部 The Kurume Medical Journal 編集部の論文
著者
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Kohno Yasuko
Department Of Pharmacology Kawanishi Pharma Research Institute Nippon Boehringer Ingelheim
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Nagasaki Nobuyuki
Department Of Pharmacology Kurume University School Of Medicine
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Tanaka Masatoshi
Department Of Comprehensive Management Matsumoto University
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TAKEDA SHIGEKO
Department of Pharmacology, Kurume University School of Medicine
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MATSUO KUMIKO
Department of Pharmacology, Kurume University School of Medicine
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