前庭単離有毛細胞のF-アクチン

概要

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Isolated living vestibular sensory cells are capable of producing self movement. After exposure to a medium containing a high concentration of potassium, type I cells show tilting of the neck portion accompanied by tilting of the hair bundle. Actin, a protein associated with the cytoskeleton and cell motility, is thought to be related to vestibular hair cell motility. The localization of F-actin in isolated vestibular hair cells has been studied with the use of FITC-labeled phallodin. In hair cells fixed by paraformaldehyde, actin filaments are distributed in the hair bundle and cuticular plate. In frozen fixed cells perfused by artificial perilymph, strong localization was found in the hair bundle and cuticular plate, as well as throughout the cytoplasm. In frozen fixed cells per-fused with artificial endolymph containing a high concentration of potassium, the staining of hair bundle and cuticular plate was less. The changes of this actin staining pattern may be closely related to the mechanism of self movement.