サポニン潅流法による前庭感覚上皮細胞の細胞骨格構造の観察

概要

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The cytoskeletal organization of the guinea pig vestibular sensory epithelial cells was investigated using the saponin perfusion method under a scanning electron microscope. The skeletal framework of a cell is composed of thin (actin or intermediate filaments) and thick filaments (microtublues). The membrane bound organelles such as the nucleus, mitochondria, endoplasmic reticulum, Golgi apparatus, secretory granules, desmosomes etc. were also well demonstrated. This facilitates investigation of three-dimensional structures of cytoskeletons as well as their complex interactions with various membranes bound organelles. Therefore, this technique may provides further information about distribution, topographic relationships and the functional role of cytoskeletons.
耳鼻咽喉科臨床学会の論文