Active uptake system for substance P carboxy-terminal heptapeptide (5-11) into a fraction from rabbit enriched in glial cells.
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概要
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In the present study, we demonstrated the existence of an active uptake system for substance P carboxy-terminal heptapeptide, (5-11)SP. When a fraction from rabbit brain enriched in glial cells was incubated with [<SUP>3</SUP>H](5-11)SP, an uptake of [<SUP>3</SUP>H](5-11)SP was observed. The uptake system has the properties of an active transport mechanism. Kinetic analysis indicated two components of [<SUP>3</SUP>H](5-11)SP uptake, one representing a high and the other a low affinity transport system. After unilateral ablation of the striatum, approximately 30% of the high affinity [<SUP>3</SUP>H](5-11)SP uptake capacity of substantia nigra slices disappeared. The subcellular distribution of the high affinity uptake indicated that [<SUP>3</SUP>H]5-hydroxy-tryptamine was taken up mostly into the P<SUB>2</SUB>B fraction (synaptosomal fraction), whereas [<SUP>3</SUP>H](5-11)SP was taken up into the P<SUB>2</SUB>A fraction (myelin fraction) to the same extent as into the P<SUB>2</SUB>B fraction. These results suggest that when SP is released from nerve terminals, it is hydrolysed into (5-11 )SP, which is in turn accumulated into glial cells as well as nerve terminals and that this high affinity uptake mechanism may play an important role in terminating the synaptic action of SP.
- 公益社団法人 日本薬理学会の論文
著者
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Inoue Atsuko
Department Of
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Yajima Haruaki
Department of Earth Sciences, Faculty of Science, Kobe University
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Nakata Yoshihiro
Department Of Biophysics Gunma University
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Segawa Tomio
Department Of Pharmacology Institute Of Pharmaceutical Sciences Hiroshima University School Of Medic
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