The Release of Substance P From Cultured Dorsal Root Ganglion Neurons Requires the Non-neuronal Cells Around These Neurons

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概要

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• Substance P is known to be released from dorsal root ganglion (DRG) neurons, but the possible involvement of non-neuronal cells in the process of substance P release is not known. During the culture period, the number of surviving DRG neurons, the total substance P content from the culture medium and DRG cells, and the total protein of DRG cells were monitored. Both the number of surviving neurons and the total substance P content decreased in a time-dependent manner, whereas the total protein synthesis was increased. The localization of substance P in small-to-medium-sized neurons was further confirmed by immunocytochemistry. Finally, the substance P release levels from the neuronal and non-neuronal enrichments were investigated by radioimmunoassay. Both partially purified DRG neurons and non-neuronal cells exhibited a weaker substance P release response to capsaicin or KCl, relative to unpurified DRG cells. The total substance P content from the partially purified DRG neurons was almost the same as that from the unpurified DRG cells, but much more than that from the partially purified DRG non-neuronal cells. These findings suggest that substance P is released from DRG neurons, and this process should require the coexistence of neurons and non-neuronal cells.

• 社団法人 日本薬理学会の論文
• 2007-11-20

著者

• Tang He-bin

  Department Of Pharmacology Graduate School Of Biomedical Sciences Hiroshima University

• Tang He-bin

  Department Of Pharmacology Division Of Clinical Pharmaceutical Sciences Programs Of Pharmaceutical S

• Tang He-bin

  広島大学 医歯薬学総合研究科薬効解析科学

• Tang He-bin

  広島大学 医歯薬学総合研究科麻酔蘇生学

• Nakata Yoshihiro

  Department of Pharmacology, Hiroshima University Graduate School of Biomedical Sciences, Japan

• Tang He-bin

  Coll. Of Pharmacy South-central Univ. For Nationalities Chn

• LI Yu-sang

  Department of Pathology, Graduate School of Biomedical Sciences, Hiroshima University

• Li Yu-sang

  Department Of Pathology Graduate School Of Biomedical Sciences Hiroshima University

• Nakata Yoshihiro

  Graduate School Of Biomedical Sciences Hiroshima Univ.

• Nakata Yoshihiro

  Department Of Biophysics Gunma University

• Nakata Yoshihiro

  Dep. Of Pharmacology Graduate School Of Biomedical Sciences Hiroshima Univ. Jpn

• Nakata Yoshihiro

  Dep. Of Pharmacology Hiroshima Univ. Graduate School Of Biomedical Sciences Jpn

• Tang He-bin

  Department Of Pharmacology College Of Pharmacy South-central University For Nationalities

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