Possible Involvement of Long Chain Fatty Acids in the Spores of Ganoderma lucidum (Reishi Houshi) to Its Anti-tumor Activity(Pharmacology)
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概要
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During our isolation of biologically active substances from the spores of Ganoderma lucidum (Reishi Houshi, G. lucidum) guided by the inhibitory activity on HL-60 cell proliferation, NMR spectroscopic and mass spectrometric data indicate the substance contains a mixture of several long chain fatty acids. Hence, in this study, we have examined the inhibitory effects of an ethanolic extract of the spores of G. lucidum as the spore extract, on the proliferation of various human cancer cell lines by comparison with several authentic long chain fatty acids. Of the fatty acids we examined nonadecanoic acid (C19:0) showed the highest inhibitory activity for HL-60 cell proliferation with IC_<50> values of 68±7μM followed by heptadecanoic acid(C17:0, 120±23μM), octa-(C18:0, 127±4μM)and hexadecanoic acids (C16:0, 132±25μM), respectively. The corresponding unsaturated fatty acids containing one double bond such as cis-10-nonadecenoic acid (C19:1), cis-9-octadecenoic acid (C18:1), cis-10-heptadecenoic acid (C17:1) and cis-9-hexadecenoic acid (C16:1) were less effective. The ethanolic extract of spores of G. lucidum were shown by annexin-V FITC/PI double staining to induce apoptosis of HL-60 cells in a similar way to cis-l0-nonadecenoic acid (C19:1). These unsaturated fatty acids probably inhibit tumor necrosis factor production induced by lipopolysaccharide in a mouse macrophage preparation. Our results suggest the spores of G. lucidum contain 19-carbon fatty acids as one of the components for characteristics of its physiological effects.
- 2008-10-01
著者
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Hirai Yuko
Department of Molecular and Internal Medicine, Hiroshima University
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Nakata Yoshihiro
Department of Pharmacology, Hiroshima University Graduate School of Biomedical Sciences, Japan
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Hide Izumi
Department of Molecular and Pharmacological Neuroscience, Graduate School of Biomedical Sciences, Hi
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Hirai Yuko
Department Of Cerebrovascular Disease National Kyushu Medical Center
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Hirai Yuko
Department Of Genetics Radiation Effects Research Foundation
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FUKUZAWA Masataka
Department of Pharmacology, Graduate School of Biomedical Sciences, Hiroshima University
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YAMAGUCHI Rie
Department of Pharmacology, Graduate School of Biomedical Sciences, Hiroshima University
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CHEN Zhiging
Iskra Industry Co., Ltd.
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SUGIMOTO Akiko
Institute of Biomaterials and Bioengineering, Tokyo Medical and Dental University
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YASUHARA Tadashi
Junior College of Tokyo University of Agriculture
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Nakata Yoshihiro
Graduate School Of Biomedical Sciences Hiroshima Univ.
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Nakata Yoshihiro
Department Of Pharmacology Graduate School Of Biomedical Sciences Hiroshima University
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Chen Zhiging
Iskra Industry Co. Ltd.
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Yamaguchi Rie
Department Of Pharmacology Graduate School Of Biomedical Sciences Hiroshima University
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Yamaguchi Rie
Department Of Environmental Biochemistry Shizuoka College Of Pharmacy
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Sugimoto Akiko
Institute Of Biomaterials And Bioengineering Tokyo Medical And Dental University
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Sugimoto Akiko
Institute For Medical And Dental Engineering Tokyo Medical And Dental University
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Hide Izumi
Department Of Pharmacology Graduate School Of Biomedical Sciences Hiroshima University
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Nakata Yoshihiro
Department Of Biophysics Gunma University
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Hide Izumi
Department Of Molecular And Pharmacological Neuroscience Graduate School Of Biomedical Sciences Hiro
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Fukuzawa Masataka
Department Of Pharmacology Graduate School Of Biomedical Sciences Hiroshima University
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Yasuhara Tadashi
Junior Coll. Of Tokyo Univ. Of Agriculture
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Nakata Yoshihiro
Dep. Of Pharmacology Hiroshima Univ. Graduate School Of Biomedical Sciences Jpn
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