Drug oxidation activities of horseradish peroxidase, myoglobin and cytochrome P-450cam reconstituted with synthetic hemes.
スポンサーリンク
概要
- 論文の詳細を見る
Drug oxidations by horseradish peroxidase (HRP), myoglobin (Mb) and cytochrome P-450<SUB>cam</SUB> (P-450<SUB>cam</SUB>) reconstituted with synthetic hemes were studied in comparison with a form of cytochrome P-450 purified from liver microsomes of polychlorinated biphenyl (PCB)-treated rats. N, N-Dimethylaniline (DMA) and 7-isopropoxycoumarin were hardly dealkylated by the heme-substituted proteins in the presence of NADPH-cytochrome <I>c</I> (P-450) reductase and NADPH, while substantial activity of this kind was observed in the presence of hydrogen peroxide or cumene hydroperoxide as oxygen donors. Specific activity varied, depending on the substrates, oxygen donors, heme derivatives and apoproteins employed. Very high levels of activity were observed in hydrogen peroxide-dependent DMA N-demethylation with HRP substituted with certain hemes. The highest level of activity was about two hundred times as high as that of rat liver cytochrome P-450. The relationship between such activity and the chemical structure of heme derivatives was discussed.
- 公益社団法人 日本薬理学会の論文
著者
-
ISHIMURA Yuzuru
Department of Biochemistry, School of Medicine, Keio University
-
Shinohara Atsuko
Department Of Epidemiology And Environmental Health Department Of Internal Medicine Juntendo Univers
-
Kato Ryuichi
Department Of Biology Graduate School Of Science Osaka University
-
Ogoshi Hisanobu
Department Of Synthetic Chemistry And Biological Chemistry Faculty Of Engineering Kyoto University
-
Ishimura Yuzuru
Department Of Biochemistry Keio University
-
Iizuka Tetsutaro
Department Of Biochemistry School Of Medicine Keio University:the Institute Of Physical And Chemical
-
SHINOHARA Atsuko
Department of Pharmacology, School of Medicine, Keio University
-
KAMATAKI Tetsuya
Department of Analytical Bio chemistry, School of Pharmacy, Hokkaido University
-
KAMATAKI Tetsuya
Department of Analytical Biochemistry, Faculty of Pharmaceutical Sciences, Hokkaido University
-
OKUDA Katsuya
Department of Chemistry, Faculty of Engineering, Technical University of Nagaoka
-
OGOSHI Hisanobu
Department of Chemistry, Faculty of Engineering, Technical University of Nagaoka
-
IIZUKA Tetsutaro
Department of Biochemistry, School of Medicine, Keio University
関連論文
- Crystallization and Preliminary X-Ray Crystallographic Studies of Thermus thermophilus HB8 MutM Protein Involved in Repairs of Oxidative DNA
- Cell-specific and Hormonally Regulated Gene Expression Directed by the CYP11B1 Gene Promoter in Rat Adrenal Cortex
- Effects of ACTH and Angiotensin II on the Novel Cell Layer without Corticosteroid-Synthesizing Activity in Rat Adrenal Cortex
- Isotopic Analysis of Calcium in Blood Plasma and Bone from Mouse Samples by Multiple Collector-ICP-Mass Spectrometry
- Precise Zn Isotopic Ratio Measurements of Human Red Blood Cell and Hair Samples by Multiple Collector-ICP-Mass Spectrometry
- MACROMOLECULAR BINDING OF CARCINOGENIC/MUTAGENIC N- HYDROXYARYLAMINES THROUGH O-ACETYLATION PATHWAY
- MECHANISM OF MUTATION AND METABOLIC ACTIVATION OF NITRO-, NITROSO-, HYDROXYLAMINO-COMPOUNDS IN NITRO-COMPOUNDS RESISTANT MUTANTS OF AMES TESTAR STRAIN (TA98NR, TA98/1,8-DNP_6)
- Effects of Drinking a Water Product with Anti-Oxidant Activities In Vitro on the Blood Levels of Biomarker Substances for the Oxidative Stress
- Studies on the Properties and Real Existence of Aqueous Solution Systems that are Assumed to Have Antioxidant Activities by the Action of "Active Hydrogen"
- EFFECTS OF Mg AND K DEFICIENCY ON MALE AND FEMALE MICE
- Indices of Lead-Exposure in Blood and Urine of Lead-Exposed Workers and Concentrations of Major and Trace Elements and Activities of SOD, GSH-Px and Catalase in Their Blood
- Distribution and Dynamic Pathway of Selenium Species in Selenium-deficient Mice Injected with ^Se-enriched Selenite
- A 13-Week Toxicity Study of Bismuth in Rats by Intratracheal Intermittent Administration
- Studies on Cytogenesis in Adult Rat Adrenal Cortex: Circadian and Zonal Variations and Their Modulation by Adrenocorticotropic Hormone
- Isotopic Analysis of Fe in Human Red Blood Cells by Multiple Collector-ICP-Mass Spectrometry
- Modulation of Ethanol-Mediated CYP2E1 Induction by Clofibrate and L-Carnitine in Rat Liver
- Formation of 2-Amino-3-methylimidazo[4,5-f]quinoline- and 2-Amino-3, 8-dimethylimidazo[4,5-f]quinoxaline-sulfamates by cDNA-expressed Mammalian Phenol Sulfotransferases
- CLONING, EXPRESSION, AND FUNCTIONAL CHARACTERIZATION OF RAT AND HUMAN PHENOL SULFOTRANSFERASES
- RAT LIVER SULFOTRANSFERASE ACTIVATING CARCINOGENIC HYDROXYMETHYL-ARENES : ITS PROPERTIES AND SEQUENCE ANALYSIS OF cDNA
- MECHANISM OF THE CHANGES IN HEPATIC DRUG METABOLIZING ENZYMES IN DIABETIC STATES
- REGULATION AND CHARACTERIZATION OF STEROID 6β-HYDROXYLASE IN RAT LIVERS
- ROLE OF GROWTH HORMONE ON THE EXPRESSION OF SEX-SPECIFIC FORMS OF CYTOCHROME P-450,P-450-MALE AND P-450-FEMALE
- STUDIES ON SEX-SPECIFIC FORMS OF CYTOCHROME P-450 IN MALE AND FEMALE RATS
- An Aspartate Aminotransferase from an Extremely Thermophilic Bacterium, Thermus thermophilus HB8^1
- Clinical course linkage among different priapism subtypes : Dilemma in the management strategies
- Crystal Structure of Thermus thermophilus HB8 UvrB Protein, a Key Enzyme of Nucleotide Excision Repair
- Cloning and characterization of the radA gene from a hyperthermophilic archaeon Pyrobaculum islandicum.
- Quantitative Analysis of Three-dimensional Fatigue Fracture Surface Reconstructed by Stereo Matching Method
- Automated Image Processing and Analysis of Fracture Surface Patterns Formed during Creep Crack Growth in Austenitic Heat-Resisting Steels with Different Microstructures
- STEREOSELECTIVE HYDROXYLATION OF MEPHENYTOIN IN HUMAN LIVERS : CLONING AND EXPRESSION OF MEPHENYTOIN HYDROXYLASE cDNAS IN YEASTS AND THE GENETIC POLYMORPHISM
- S-5 CURRENT TOPICS OF SPECIES SPECIFICITY IN DRUG TOXICITY-METABOLIC ASPECT
- Changes in Cerebral Oxygen Saturation and Blood Flow During Hypoxic Gas Ventilation Therapy in HLHS and CoA/IAA Complex With Markedly Increased Pulmonary Blood Flow
- TOXICOLOGICAL SIGNIFICANCE OF A FORM OF CYTOCHROME P-450 HIGHLY PURIFIED FROM LIVER MICROSOMES OF PCBTREATED RATS : CATALYTIC AND IMMUNOCHEMICAL EXAMINATIONS OF A HIGH SPIN FORM OF CYTOCHROME P-448
- The Role of Glutathione in the Activation and Detoxication of a Naturally Occurring Mutagen-Carcinogen, Trp-P-2 (Regular Presentations) (Proceedings of the 10 th Symposium on Environmental Pollutants and Toxicology)
- MECHANISMS INVOLVED IN THE COVALENT BINDING OF A TRYPTOPHAN-PYROLYSATE PROMUTAGEN, TRP-P-2,TO DNA
- STUDIES ON CYTOCHROME P-450 RESPONSIBLE FOR THE OCCURRENCE OF SEX DIFFERENCE OF DRUG METABOLISM IN THE RAT
- A SENSITIVE METHOD FOR THE DETERMINATION OF BIPHENYL HYDROXYLASE ACTIVITY AND IT'S TOXICOLOGICAL APPLICATION
- MECHANISMS OF METABOLIC ACTIVATION OF A TRYPTOPHAN PYROLYSATE, 3-AMINO-1-METHYL-5H-PYRIDO [4,3-b] INDOLE
- PURIFICATION AND PROPERTIES OF CYTOCHROME P-450 AND NADPH-CYTOCHROME C (P-450) REDUCTASE FROM MICROSOMES OF HUMAN LIVERS
- STUDIES ON THE METABOLIC ACTIVATION AND THE MECHANISM OF TOXICITY OF DRUGS V. REDUCTION OF N-HYDROXY DERIVATIVE OF AN AROMATIC AMINE, 2-ACETYLAMINOFLUORENE BY CYTOCHROME P-450 (The 6th Meeting for the Study of Toxic Effect)
- DEGRADATION OF CYTOCHROME P-450 ASSOCIATED WITH METABOLIC ACTIVATION OF CARBON TETRACHLORIDE (The 5th Meeting for the Study of Toxic Effect)
- Efficacy of a 14-day course of oral ciprofloxacin therapy for acute uncomplicated pyelonephritis
- WSII-3 Visualization of Oxidative Stress and Cell Dysfunction in Organ Microcirculation
- ALTERATIONS IN MITOCHONDRIAL FUNCTION BY SUPPRESSION OF CARBON MONOXIDE IN CULTURED RAT HEPATOCYTES
- CARBON MONOXIDE : AN ENDOGENOUS MODULATOR OF HEPATOBILIARY FUNCTIONS
- Determination of Selenium Concentrations in Human Blood Plasma by Microwave-Induced Plasma Mass Spectrometry
- Nerve injury-related erectile dysfunction following nerve-sparing radical prostatectomy : A novel experimental dissection model
- Alteration of glial cell line-derived neurotrophic factor family receptor alpha-2 mRNA expression and its co-expression with neuronal nitric oxide synthase in pelvic ganglia following unilateral cavernous nerve injury
- Outcome analysis for conservative management of Peyronie's disease
- Erectile dysfunction following nerve-sparing radical retropubic prostatectomy and its treatment with sildenafil
- Erosion of a penile prosthesis due to an indwelling urethral catheter as a late complication
- The correlation between penile tumescence measured by the erectometer and penile rigidity by the RigiScan
- A Case of Chronic Infantile Neurological Cutaneous Articular (CINCA) Syndrome with Marked Growth Disturbance
- CYTOCHROME P-450 CONTAINING PROTEIN-PROTEIN INTERACTIONS IN BOVINE ADRENOCORTICAL MITOCHONDRIA
- Fractal Dimension of Grain-boundary Fracture for Characterization of High-temperature Fracture in Heat-resistant Alloys
- Quantitative Evaluation of the Impact Fracture Surfaces of SS400 Steel by the Three-dimensional Geometrical Analysis
- X-Ray Crystal Structure and Catalytic Properties of Thr252Ile Mutant of Cytochrome P450cam: Roles of Thr252 and Water in the Active Center^1
- Fractal Analysis of Fracture Surfaces in Materials Crept at High Temperatures or Fatigued by Repeated Bending
- Change in the Fracture Surface Pattern in the Fracture Process of SUS631 Stainless Steel Fatigued by Repeated Bending
- Characterization of Insulin-Dependent Diabetes Mellitus Induced by a New Variant (DK-27) of Encephalomyocarditis Virus in DBA/2 Mice
- The sequential multistep leukocyte-endothelial interactions are not applicable in the rat perfused lung microcirculation
- TOXICOKINETICS:ITS SIGNIFICANCE AND PRACTICAL PROBLEMS
- Detection of a Hydroperoxy-Heme Species Formed in the Reaction of Ferric Myoglobin with H_2O_2
- Further Studies on the Persistence of Neonatal Androgen Imprinting on Sex-Specific Cytochrome P-450, Testosterone and Drug Oxidations
- Effects of Hypophysectomy and Growth Hormone Treatment on Sex-Specific Forms of Cytochrome P-450 in Relation to Drug and Steroid Metabolisms in Rat Liver Microsomes
- METABOLIC FATE OF FEPRAZONE
- EFFECTS OF α- AND β-ADRENERGIC BLOCKERS ON CHLORPROMAZINE-INDUCED ELEVATION OF PLASMA GLUCOSE AND CYCLIC AMP IN FED MICE
- EFFECT OF CHLORPROMAZINE ON INSULIN RELEASE IN MICE: COMPARISON BETWEEN IN VIVO AND IN VITRO
- AN UNUSUAL METABOLITE OF TESTOSTERONE BY CYTOCHROME P-450
- Anthralin, a Non-TPA Type Tumor Promoter, Synergistically Enhances Phorbol Ester-Caused Prostaglandin E2 Release from Primary Cultured Mouse Epidermal Cells
- Inhibitory Regulation of Serum Factor(s)-Caused Ornithine Decarboxylase Induction by the Protein Kinase C System in A431 Human Epidermoid Carcinoma Cells
- Mechanism of Thyroxine-induced Increase in Steroid Δ4-Reductase Actiyity in Male Rats
- Effect of Adrenalectomy on the Substrate Interaction with Cytochrome P-450 in the Hydroxylation of Steroid Hormones by Liver Microsomes
- Superoxide, NO and CO in liver microcirculation : Physiology and pathophysiology
- PARTIAL AGONISTIC ACTION OF MORPHINE IN THE RAT VAS DEFERENS
- TOXICITY OF PLATINUM COMPLEXES WITH ANTITUMOR ACTIVITY (I)
- INHIBITION BY SKF 525-A OF 7-ETHOXYCOUMARIN O-DEETHYLATION IN MICROSOMAL AND THE RECONSTITUTED MONOOXYGENASE SYSTEMS FROM PCB-TREATED RAT LIVERS
- SA-06-2(013) Size Distribution of Creep Cracks and Fracture Mechanisms in High-Temperature Materials(Flaw Assessment at Elevated Temperature 1)
- PREVENTION OF CATECHOLAMINE RELEASE FROM ADRENAL CHROMAFFIN CELLS BY PHOSPHOLIPASE A2- AND LIPOXYGENASE-INHIBITORS
- Activation of Phorbol Ester Responsive Form of Protein Kinase CC in Association with Ca^ : Induced Differentiation of Primary Cultured Mouse Epidermal Cells
- The Heme Oxygenase-Carbon Monoxide System as a Regulator of Microvascular Function
- Effect of Androgen and Estrogen on the Hydroxylation of Steroid Hormones by Rat Liver Microsomes
- Expression and Functional Characterization of a Rat Sulfotransferase (ST1A1)cDNA for Sulfations of Phenolic Substrates in COS-1 Cells
- Microstructures of Cutting Chips of SUS430 and SUS304 Steels, and NCF 750 and 6061-T6 Alloys
- THE APPARENT LOSS OF CYTOCHROME P-450 ASSOCIATED WITH METABOLIC ACTIVATION OF CARBON TETRACHLORIDE
- Plasma cyclic AMP in the pethidine-dependent rat.
- Hepatic level of mRNA in relation to polymorphism and strain difference in expression of cytochrome P-450g.
- Effect of Systemic L-Arginine Administration on Hemodynamics and Nitric Oxide Release in Man.
- Dexamethasone Inhibits Nitric Oxide Synthase mRNA Induction by Interleukin-1.ALPHA. and Tumor Necrosis Factor-.ALPHA. in Vascular Smooth Muscle Cells.
- Nitric Oxide Synthase mRNA in Endothelial Cells: Synergistic Induction by Interferon-.GAMMA., Tumor Necrosis Factor-.ALPHA. and Lipopolysaccharide and Inhibition by Dexamethasone.
- Drug oxidation activities of horseradish peroxidase, myoglobin and cytochrome P-450cam reconstituted with synthetic hemes.
- Regulations of Male-Dominant P-450Md mRNA in Rat Liver by Hormonal Factors and Xenobiotics.
- Inhibition of teleocidin-caused epidermal ornithine decarboxylase induction by phospholipase A2-, cyclooxygenase- and lipoxygenase-inhibitors.
- Synergistic Stimulation of Prostaglandin E2 Release by Epidermal Growth Factor and a Tumor Promoter Anthralin from Primary Cultures of Mouse Epidermal Cells.
- Inhibition of 12-O-tetradecanoylphorbol-13-acetate-induced increase in vascular permeability in mouse skin by lipoxygenase inhibitors.
- Comparison of some biochemical properties of epidermis in tumor promotion-susceptible and -resistant strains of mice.
- Induction of cytochrome P-450c and P-450d by metyrapone in the primary culture of rat hepatocytes.
- Nitric Oxide in Vascular Remodeling.
- Beneficial Circulatory Effect of L-Arginine.
- Tension-induced release of endothelium-derived relaxing factor; Possible role in establishment of desensitization of norepinephrine-induced contraction in rat aorta.