Characterization of Insulin-Dependent Diabetes Mellitus Induced by a New Variant (DK-27) of Encephalomyocarditis Virus in DBA/2 Mice

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概要

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• A murine diabetes mellitus induced with a new diabetogenic variant (DK-27) which we isolated from the M variant of the encephalomyocarditis (EMC) virus was characterized. Male DBA/2 mice (9.5 weeks old) were infected with various infectious doses of DK-27 intraperitoneally. Blood glucose and insulin levels were examined in association with the viral replication. Pancreatic pathology and hormone contents and stable hemoglobin A1c (St-A1c) levels were also examined on the final day of observation (35 days of post-infection). In infected mice, blood glucose levels rapidly elevated at 72 hr, slightly decreased between 7 and 10 days and finally became sustained hyperglycemia. On the other hand, blood insulin levels elevated at 48 hr, promptly decreased, and subsequently became sustained hypoinsulinemia. Viral replication in pancreases reached the highest titers at 48 hr and rapidly disappeared with all infectious doses used. Pancreatic insulin contents in infected mice were not detectable, and glucagon contents were not affected. In pathological examination, atrophy of islets and marked diminution of B-cells were observed, and A-cells occupied the major part of an infected islet. St-A1c levels reflected lasting hyperglycemia. These findings show that DK-27 causes insulin-dependent diabetes mellitus by the specific and direct destruction of pancreatic B-cells in susceptible mice. Such a diabetic model mouse will be useful for therapeutic studies.

• 社団法人　日本実験動物学会の論文

著者

• KATO Ryuichi

  Department of Biology,Graduate School of Science,Osaka University

• Kato R

  Division Of Chemotherapy Pharmaceutical Institute School Of Medicine Keio University

• Kato Ryuichi

  Department Of Biology Graduate School Of Science Osaka University

• SETO Yoshiko

  Division of Chemotherapy, Pharmaceutical Institute, School of Medicine, Keio University

• DAN Katsuaki

  Division of Chemotherapy, Pharmaceutical Institute, School of Medicine, Keio University

• FUJITA Haruhisa

  Division of Chemotherapy, Pharmaceutical Institute, School of Medicine, Keio University

• Takei Izumi

  Internal Medicine School Of Medicine Keio University

• Dan Katsuaki

  Division Of Chemotherapy Pharmaceutical Institute School Of Medicine Keio University

• Seto Yoshiko

  Division Of Chemotherapy Pharmaceutical Institute School Of Medicine Keio University

• FUJITA Tomonobu

  Division of Cellular Signaling, Institute for Advanced Medical Research, Keio University, School of

• Fujita Tomonobu

  Division Of Cellular Signaling Institute For Advanced Medical Research Keio University School Of Med

• Fujita Tomonobu

  Division Of Chemotherapy Pharmaceutical Institute School Of Medicine Keio University

• ASABA Yoshiaki

  Internal Medicine, Urawa Municipal Hospital

• Asaba Yoshiaki

  Internal Medicine Urawa Municipal Hospital

• Fujita Haruhisa

  Division Of Chemotherapy Pharmaceutical Institute School Of Medicine Keio University

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