Novel Hemoplasma Species Detected in Free-Ranging Sika Deer (Cervus nippon)
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概要
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Hemoplasma infections in wild ungulates have not been reported yet in Japan. We examined presence of hemoplasmas in blood samples collected from 147 sika deer (Cervus nippon) in the Iwate prefecture by real-time PCR, and found 13 (9%) were positive. Almost entire region of the 16S rRNA gene of the representative strains from positive samples was amplified by conventional PCR. The nucleotide sequences of the 16S rRNA gene were further determined and compared with those of other hemoplasmas. Our examinations first revealed the presence of two distinct hemoplasma species in sika deer, which are previously not described. One of them was closely related to M. ovis by the 16S rRNA sequence analysis, but was found distinct by comparison of the RNase P RNA gene sequences. Pathogenicity of these two hemoplasma species in sika deer is currently unknown.
著者
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OBARA Hisato
Department of Veterinary Microbiology, School of Veterinary Medicine, Faculty of Agriculture, Iwate
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FUJIHARA Masatoshi
Department of Veterinary Microbiology, School of Veterinary Medicine, Faculty of Agriculture, Iwate
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WATANABE Yusaku
Department of Veterinary Microbiology, School of Veterinary Medicine, Faculty of Agriculture, Iwate
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HARASAWA Ryô
Department of Veterinary Microbiology, School of Veterinary Medicine, Faculty of Agriculture, Iwate
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松原 和衛
岩手大学
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Harasawa Ryo
Department Of Veterinary Medicine Faculty Of Agriculture Iwate University
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MATSUBARA Kazuei
Department of Animal Sciences, Faculty of Agriculture, Iwate University
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YAMAUCHI Kiyoshi
Research Institute for Environmental Sciences and Public Health of Iwate Prefecture
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Matsubara Kazuei
Department Of Animal Science Faculty Of Agriculture Iwate University
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松原 和衛
岩手大学大学院農学研究科
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Matsubara Kazuei
Graduate School Of Agriculture Iwate University
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