Mycoplasma ovis Detected in Free-Living Japanese Serows, Capricornis crispus
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概要
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Nineteen blood samples collected from free-ranging wild Japanese serows, Capricornis crispus, between 2006 and 2008 in Iwate prefecture were examined for the hemoplasma infection by real-time PCR targeting the 16S rRNA gene. Five (26.3%) out of the 19 samples were positive in real-time PCR with an average melting temperature at 85.18°C. The positive samples in the real-time PCR were reconfirmed by conventional PCR, and one of them was successful for direct DNA sequencing. The nucleotide sequence of the 16S rRNA gene of the representative stain was identical to that of Mycoplasma ovis. This was the first demonstration of hemotropic mycoplasma infections among the free-living Japanese serows in Japan.
著者
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Harasawa Ryo
Department Of Veterinary Medicine Faculty Of Agriculture Iwate University
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Matsubara Kazuei
Department Of Animal Science Faculty Of Agriculture Iwate University
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Nishimura Takashi
Department Of Animal Science Faculty Of Agriculture Iwate University
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Sato Makoto
Department Of Applid Chemistry Graduate School Of Engineering Tohoku University
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WATANABE Yusaku
Department of Veterinary Medicine, Faculty of Agriculture, Iwate University, Morioka 020–8550, Japan
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NAGAI Kazuya
Cryobiofrontier Research Center, Faculty of Agriculture, Iwate University
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NISHIZAWA Ikuo
Department of Veterinary Microbiology, Faculty of Agriculture, Iwate University
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OHTAKE Yoshihiro
Department of Veterinary Microbiology, Faculty of Agriculture, Iwate University
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